Ferrari S, Tarugi P, Drusiani E, Calandra S, Fregni M
Istituto di Chimica Biologica, Università di Modena, Italy.
Gene. 1987;60(1):39-46. doi: 10.1016/0378-1119(87)90211-3.
The nucleotide (nt) sequence analysis of a full-length cDNA for chick apolipoprotein AI (Apo-AI) shows an open reading frame (ORF) of 792 nt, coding for a 264-aa protein. RNase mapping and sequence analysis of the 3' end show that apo-AI mRNA consists of at least two different species of 985 and 996 nt, respectively. During the embryonic life of the chick apo-AI mRNA is found in high concentration only in the liver, while its level in the intestine, the major Apo-AI producing organ in the adult, becomes significant only after hatching. This switch from liver to intestine, as primary site of apo-AI mRNA synthesis, takes place about ten days after hatching. The developmental control of the tissue levels of apo-AI mRNA is particularly evident in the skeletal muscle, where this mRNA species is present at high level only immediately after hatching. Preliminary evidence suggests that the time-limited rise in muscle apo-AI mRNA might be due to an increased rate of transcription.
对鸡载脂蛋白AI（Apo-AI）全长cDNA的核苷酸（nt）序列分析显示，其开放阅读框（ORF）为792 nt，编码一个264个氨基酸的蛋白质。对3'端的核糖核酸酶定位和序列分析表明，apo-AI mRNA分别由至少两种不同的985 nt和996 nt的mRNA组成。在鸡的胚胎期，仅在肝脏中发现高浓度的apo-AI mRNA，而在成体中主要产生Apo-AI的器官——肠道中，其水平仅在孵化后才显著升高。从肝脏到肠道作为apo-AI mRNA合成主要位点的这种转变发生在孵化后约十天。apo-AI mRNA组织水平的发育调控在骨骼肌中尤为明显，在骨骼肌中，这种mRNA仅在孵化后立即高水平存在。初步证据表明肌肉中apo-AI mRNA的限时升高可能是由于转录速率增加所致。
