School of Food Science and Engineering, Foshan University, Foshan 528000, China.
School of Food Science and Engineering, Foshan University, Foshan 528000, China; Institute of Environment and Food Safety, Wuhan Academy of Agricultural Science, Wuhan 430065, China.
Food Chem. 2019 Nov 15;298:125034. doi: 10.1016/j.foodchem.2019.125034. Epub 2019 Jun 17.
A wash-free and label-free colorimetric biosensor for the amplified detection of aflatoxin B1 (AFB1) has been constructed by the integration of an ingenious hairpin DNA probe with exonuclease III (Exo III)-assisted signal amplification. The presence of the AFB1 activates the continuous cleavage reactions by Exo III toward a hairpin probe, resulting in the autonomous accumulation of numerous free G-quadruplex sequences, which can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HO to produce a colorimetric response. The naked-eye biosensor is ultrasensitive, enabling the visual detection of trace amounts of AFB1 as low as 1 pM without instrumentation. The sensor is robust and can work even when challenged with complex sample matrices such as peanut samples. With the advantages of simple operation, wash-free and label-free format, visible and intuitive output, and low cost, the naked-eye based colorimetric biosensor is expected to have potential applications for in-field detection of AFB1.
一种免洗、无标记的比色生物传感器，用于放大检测黄曲霉毒素 B1（AFB1），通过巧妙的发夹 DNA 探针与外切酶 III（Exo III）辅助信号放大的集成而构建。存在 AFB1 会激活 Exo III 对发夹探针的连续切割反应，导致大量游离 G-四链体序列的自主积累，这些序列可以通过 HO 催化 3,3',5,5'-四甲基联苯胺（TMB）的氧化产生比色反应。肉眼生物传感器具有超灵敏性，无需仪器即可目视检测低至 1 pM 的痕量 AFB1。该传感器坚固耐用，即使在花生等复杂样本基质的挑战下也能正常工作。基于肉眼的比色生物传感器具有操作简单、免洗、无标记格式、可见直观的输出以及低成本等优点，有望在现场检测 AFB1 方面具有应用潜力。
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