消旋美沙酮作为头颈癌细胞系中化疗药物的增强剂
DL-Methadone as an Enhancer of Chemotherapeutic Drugs in Head and Neck Cancer Cell Lines.
作者信息
Landgraf Veronika, Griessmann Matthias, Roller Jeanette, Polednik Christine, Schmidt Marianne
机构信息
Department of Otorhinolaryngology, University of Wuerzburg, Wuerzburg, Germany.
Department of Otorhinolaryngology, University of Wuerzburg, Wuerzburg, Germany
出版信息
Anticancer Res. 2019 Jul;39(7):3633-3639. doi: 10.21873/anticanres.13511.
BACKGROUND/AIM: The aim of this study was to analyze the effect of DL-methadone on enhancing the action of the chemotherapeutic drugs cisplatin, doxorubicin, 5-fluoruracil (5-FU) and paclitaxel on head and neck squamous carcinoma (HNSCC) cell lines.
MATERIALS AND METHODS
The chemotherapeutic drugs were applied alone or in combination with DL-methadone and cytotoxicity was analyzed by XTT assays. Expression of the μ-opioid receptor and the drug transporter p-glycoprotein were analyzed by qRT-PCR.
RESULTS
The effect of DL-methadone strongly depended on the respective chemotherapeutic agent. The basic expression of the μ-opioid receptor was not associated with the effect of DL-methadone, rather its induction by chemotherapeutic drugs. Expression or expression induction of p-glycoprotein was higher in weak-responder cell lines.
CONCLUSION
Enhancement of the toxicity of chemotherapeutic drugs by DL-methadone depends on the drug and on the cell line used.
背景/目的：本研究旨在分析消旋美沙酮对增强化疗药物顺铂、阿霉素、5-氟尿嘧啶（5-FU）和紫杉醇对头颈部鳞状细胞癌（HNSCC）细胞系作用的影响。
材料与方法
化疗药物单独应用或与消旋美沙酮联合应用，通过XTT法分析细胞毒性。采用qRT-PCR分析μ-阿片受体和药物转运蛋白P-糖蛋白的表达。
结果
消旋美沙酮的作用强烈依赖于各自的化疗药物。μ-阿片受体的基础表达与消旋美沙酮的作用无关，而是与其被化疗药物诱导有关。P-糖蛋白的表达或表达诱导在低反应性细胞系中较高。
结论
消旋美沙酮增强化疗药物毒性的作用取决于所用药物和细胞系。
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