Jiang Min-Shan, Yang Ting, Xu Xiao-Li, Zhang Xue-Dian, Li Feng
Shanghai Key Laboratory of Contemporary Optics System, College of Optical-Electrical and Computer Engineering, University of Shanghai for Science and Technology, Shanghai, China.
Microsc Res Tech. 2019 Sep;82(9):1621-1627. doi: 10.1002/jemt.23328. Epub 2019 Jul 2.
Microscopic imaging of uneven surfaces is difficult because of the limited depth of field. In this study, we developed a rapid auto-focus method for uneven surfaces based on image fusion. The Prewitt operator was used to detect the vertical edges of the images. Then, the focus position was theoretically calculated using a Gaussian function, and image fusion was applied to obtain the final in-focus image. An experiment was designed to verify the developed method. The results revealed that this method is effective for printed circuit boards.