在大肠杆菌中产生的白细胞介素2-绿脓杆菌外毒素嵌合蛋白的细胞毒性活性。
Cytotoxic activity of an interleukin 2-Pseudomonas exotoxin chimeric protein produced in Escherichia coli.
作者信息
Lorberboum-Galski H, FitzGerald D, Chaudhary V, Adhya S, Pastan I
机构信息
Laboratory of Molecular Biology, National Cancer Institute, Bethesda, MD 20892.
出版信息
Proc Natl Acad Sci U S A. 1988 Mar;85(6):1922-6. doi: 10.1073/pnas.85.6.1922.
A cDNA clone for human interleukin 2 (IL-2) has been fused to the 5' end of a modified Pseudomonas exotoxin (PE) gene that lacks the sequences encoding the cell recognition domain. The chimeric protein IL-2-PE40 was produced in Escherichia coli. It was extremely toxic to IL-2 receptor-positive cells but had no measurable effect on cells lacking the IL-2 receptor. IL-2-PE40 might be a useful cytotoxic agent in the treatment of diseases involving IL-2 receptor-positive cells and in the treatment of allograft rejection.
人白细胞介素2（IL-2）的一个cDNA克隆已与修饰的绿脓杆菌外毒素（PE）基因的5'端融合，该外毒素基因缺乏编码细胞识别结构域的序列。嵌合蛋白IL-2-PE40在大肠杆菌中产生。它对IL-2受体阳性细胞具有极强的毒性，但对缺乏IL-2受体的细胞没有可测量的影响。IL-2-PE40在治疗涉及IL-2受体阳性细胞的疾病和同种异体移植排斥反应中可能是一种有用的细胞毒性药物。
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