McKenna P B, Charleston W A
Palmerston North Animal Health Laboratory, New Zealand.
Vet Parasitol. 1988 Jan;26(3-4):215-27. doi: 10.1016/0304-4017(88)90090-8.
A method for the mass recovery of S. gigantea sporocysts from cat faeces involving homogenisation and centrifugation in water, passage through 250- and 53-microns sieves and floatation in 1.2 SG NaCl solution, is described. An examination of the various processes involved in this procedure showed that the greatest yields were obtained when a proportion of faeces to floatation medium of 1:20 and centrifugation at 6000 X g for at least 5 min was used. Ninety-six per cent of the sporocysts recovered were obtained from the first centrifugation in aqueous NaCl solution (SG 1.2). Although neither sieving nor additional washing of homogenised samples prior to floatation significantly affected sporocyst recovery, both reduced the amount of debris present. A considerable reduction in the amount of debris resulted from feeding infected cats on tinned fish rather than tinned meat. The addition of CCl4 to the NaCl solution also improved sporocyst purity but with a marked reduction in the numbers recovered.
本文描述了一种从猫粪便中大量回收巨型隐孢子虫卵囊的方法，该方法包括在水中进行匀浆和离心、通过250微米和53微米筛网以及在1.2比重的氯化钠溶液中浮选。对该程序中涉及的各种过程进行检查后发现，当粪便与浮选介质的比例为1:20且在6000×g下离心至少5分钟时，可获得最高产量。回收的卵囊中96%是在氯化钠水溶液（比重1.2）中首次离心时获得的。尽管在浮选前对匀浆样品进行筛分或额外洗涤对卵囊回收率没有显著影响，但两者都减少了存在的碎片量。用罐装鱼而不是罐装肉喂养受感染的猫可使碎片量显著减少。向氯化钠溶液中添加四氯化碳也提高了卵囊纯度，但回收的数量明显减少。
