Soudon J, Vilarem M J, Caron de Fromentel C, Gras M P, Larsen C J
I.N.S.E.R.M., Institut de Recherches sur les Maladies du sang, Hôpital Saint-Louis, Paris.
C R Acad Sci III. 1988;306(2):35-8.
Expression of the gene encoding the nuclear phosphoprotein p53 (a proto-oncogene classified in the same functional family as c-myc and E1a adenovirus gene) was examined in a human T-cell leukemia (KE-37R cell line). No p53 (or a modified product) could be detected by immunoprecipitation with monoclonal antibodies P Ab 421 and P Ab 122 in KE-37R cell extracts, and no p53-specific RNA was characterized by Northern blot analysis. Southern blot using a murine p53 cDNA clone as a probe, did not reveal any gross rearrangement in the structure of the gene. However, this molecular probe was not suited for investigating the 5' end of the gene which contains the promoter and the non coding exon 1. It is interesting to notice that in KE-37R cells, c-myc has been activated by a t(8; 14) (q24; q11) translocation, suggesting that the c-myc product might substitute to some functions normally requiring p53.
在人T细胞白血病（KE - 37R细胞系）中检测了编码核磷蛋白p53（一种原癌基因，与c - myc和E1a腺病毒基因属于同一功能家族）的基因表达。用单克隆抗体P Ab 421和P Ab 122对KE - 37R细胞提取物进行免疫沉淀，未检测到p53（或修饰产物），Northern印迹分析也未鉴定出p53特异性RNA。以鼠p53 cDNA克隆为探针进行Southern印迹分析，未发现该基因结构有任何明显重排。然而，这种分子探针不适用于研究包含启动子和非编码外显子1的基因5'端。有趣的是，在KE - 37R细胞中，c - myc已通过t(8; 14) (q24; q11)易位被激活，这表明c - myc产物可能替代了一些通常需要p53的功能。
