Shenzhen Blood Centre, Shenzhen, China.
Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
BMC Infect Dis. 2019 Jul 3;19(1):574. doi: 10.1186/s12879-019-4215-9.
Blood donor plasma samples were detected by the Ultrio Plus NAT system for HBV, HCV and HIV-1 in Shenzhen blood center, China. Reactive samples underwent further discriminatory testing of a single virus by the same methodology. A large number of cases of non-discriminated reactive (NDR) donors were found, leaving potential risk of transmitting HBV if not deferrals. This study identified those non-discriminated samples.
The NDR plasma samples from blood donation screening were detected and classified by additional molecular and serological tests. Molecular characterizations of DNA+ NDR were determined by sequencing analysis.
A number of 259 (0.21%) NDR plasma samples from screening of 123,280 eligible blood donors were detected, which presented a higher rate (91.1%) of anti-HBc reactivity and nearly half (46.7%) of HBV DNA+ that classified as occult HBV infection (OBI). Most OBI strains were wild-type HBV, but some substitutions V168A, S174 N, V177A, Q129R/L/H, G145A/R in S region of genotype B (OBI) and T47K/V/A, P49H/L, Q101R/H/K, S174 N, L175S, V177A, T118 M/R/K, G145R/A/K/E, R160K/N in S region of genotype C (OBI) strains were identified in high frequency.
Nearly half of NDR blood samples were identified as OBI, in which a number of important mutations were detected. NDR donation might have potential risk for HBV transmission, but need to be further investigated.
在中国深圳血液中心,采用 Ultrio Plus NAT 系统检测血液捐献者血浆样本中的乙肝病毒(HBV)、丙型肝炎病毒(HCV)和人类免疫缺陷病毒 1 型(HIV-1)。对呈反应性的样本采用相同方法进行单一病毒的进一步鉴别检测。发现大量非鉴别反应性(NDR)供体样本,若不进行延期,存在传播 HBV 的潜在风险。本研究对这些非鉴别样本进行了鉴定。
通过额外的分子和血清学检测对献血筛查中的 NDR 血浆样本进行检测和分类。通过测序分析确定 DNA+NDR 分子特征。
从 123280 名合格献血者的筛查中检测到 259 份(0.21%)NDR 血浆样本,其抗 HBc 反应率较高(91.1%),近一半(46.7%)HBV DNA+为隐匿性乙肝感染(OBI)。大多数 OBI 株为野生型 HBV,但在 B 型(OBI)的 S 区发现了一些取代 V168A、S174N、V177A、Q129R/L/H、G145A/R 和在 C 型(OBI)的 S 区发现了 T47K/V/A、P49H/L、Q101R/H/K、S174N、L175S、V177A、T118M/R/K、G145R/A/K/E、R160K/N 的高频突变。
近一半的 NDR 血液样本被鉴定为 OBI,其中发现了一些重要的突变。NDR 献血可能存在 HBV 传播的潜在风险,但需要进一步研究。