Okutomi T, Yamazaki M
Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
Cancer Res. 1988 Apr 1;48(7):1808-11.
gamma-Interferon (IFN-gamma) was shown to amplify cytotoxin release from murine bone marrow-adherent macrophages in response to an animal lectin as a second stimulus. This stimulation of cytotoxin release was demonstrated both in vitro and in vivo and was maximal 2 h after treatment with IFN-gamma. No change in expression of surface antigens was detected on bone marrow-adherent cells treated with IFN-gamma. IFN-gamma also augmented production of cytotoxin from bone marrow-adherent cells that had been cultured for several days. This cytotoxin was inhibited by rabbit anti-tumor necrosis factor serum and had a molecular weight of 70,000. These findings showed that both freshly isolated and cultured bone marrow macrophages could release cytotoxin and that cytotoxin production in response to a second stimulus was augmented by IFN-gamma. The results also suggest that IFN-gamma can activate all macrophages in vivo, irrespective of their states of maturation and differentiation.
γ-干扰素（IFN-γ）已被证明可在作为第二种刺激物的动物凝集素作用下，增强小鼠骨髓黏附巨噬细胞的细胞毒素释放。这种对细胞毒素释放的刺激在体外和体内均得到证实，且在用IFN-γ处理后2小时达到最大值。在用IFN-γ处理的骨髓黏附细胞上未检测到表面抗原表达的变化。IFN-γ还增强了培养数天的骨髓黏附细胞的细胞毒素产生。这种细胞毒素被兔抗肿瘤坏死因子血清抑制，分子量为70,000。这些发现表明，新鲜分离的和培养的骨髓巨噬细胞均可释放细胞毒素，且IFN-γ可增强对第二种刺激的细胞毒素产生。结果还表明，IFN-γ可在体内激活所有巨噬细胞，无论其成熟和分化状态如何。
