Hedrick J L, Katagiri C
Department of Biochemistry and Biophysics, University of California, Davis 95616.
J Exp Zool. 1988 Jan;245(1):78-85. doi: 10.1002/jez.1402450112.
The antigenic relationship of the egg jelly coat glycoproteins from Bufo japonicus japonicus and Xenopus laevis laevis was investigated using agar double diffusion methods. The presence of ligands in the jelly coats for the cortical granule lectin from X.l. laevis eggs was also investigated. Anti-jelly serum for both anuran species crossreacted with the jelly coat from the other species with precipitin patterns of identity. Each egg jelly coat of both species contained two ligands for the cortical granule lectin. Although the ligands in the two different jelly coats appeared to react with the lectin in a pattern of identity, the species ligands were antigenically distinguishable using anti-Xenopus jelly serum. The observations that the two anuran egg jelly coats were antigenically related and that they both contained ligands for the X.l. laevis cortical granule lectin was interpreted in terms of fertilization mechanisms in the two different species. In addition, these observations bring into question the currently accepted phylogenetic relationship of B.j. japonicus and X.l. laevis.
利用琼脂双向扩散法研究了日本林蛙和非洲爪蟾卵胶膜糖蛋白的抗原关系。还研究了非洲爪蟾卵皮质颗粒凝集素在卵胶膜中配体的存在情况。两种无尾两栖类动物的抗卵胶血清与另一种动物的卵胶膜发生交叉反应，沉淀模式相同。两种动物的每个卵胶膜都含有两种皮质颗粒凝集素的配体。虽然两种不同卵胶膜中的配体似乎以相同模式与凝集素发生反应，但使用抗非洲爪蟾卵胶血清可在抗原上区分物种配体。两种无尾两栖类动物的卵胶膜具有抗原相关性且都含有非洲爪蟾皮质颗粒凝集素的配体这一观察结果，从两种不同物种的受精机制方面进行了解释。此外，这些观察结果对目前所接受的日本林蛙和非洲爪蟾的系统发育关系提出了质疑。
