Imagerie et Modélisation en Neurobiologie et Cancérologie (IMNC), CNRS, Univ Paris Sud, Université Paris-Saclay, Orsay, France.
Université de Paris, IMNC, Orsay, France.
J Biophotonics. 2019 Nov;12(11):e201900111. doi: 10.1002/jbio.201900111. Epub 2019 Jul 23.
The surgical outcome of brain tumor resection and needle biopsy is significantly correlated to the patient's prognosis. Brain tumor surgery is limited to resecting the solid portion of the tumor as current intraoperative imaging modalities are incapable of delineating infiltrative regions. For accurate delineation, in situ tissue interrogation at the submicron scale is warranted. Additionally, multimodal detection is required to remediate the genetically and molecularly heterogeneous nature of brain tumors, notably, that of gliomas, meningioma and brain metastasis. Multimodal detection, such as spectrally- and temporally-resolved fluorescence under one- and two-photon excitation, enables characterizing tissue based on several endogenous optical contrasts. In order to assign the optically-derived parameters to different tissue types, construction of an optical database obtained from biopsied tissue is warranted. This report showcases the different quantitative and semi-quantitative optical markers that may comprise the tissue discrimination database. These include: the optical index ratio, the optical redox ratio, the relative collagen density, spectrally-resolved fluorescence lifetime parameters, two-photon fluorescence imaging and second harmonic generation imaging.
脑肿瘤切除和针吸活检的手术结果与患者的预后有显著的相关性。脑肿瘤手术仅限于切除肿瘤的实体部分，因为目前的术中成像方式无法描绘出浸润区域。为了进行准确的描绘，有必要在亚微米尺度上对组织进行原位检测。此外，还需要进行多模态检测，以解决脑肿瘤（特别是神经胶质瘤、脑膜瘤和脑转移瘤）在基因和分子上的异质性问题。多模态检测，如单光子和双光子激发下的光谱和时间分辨荧光，能够基于几种内源性光学对比来对组织进行特征描述。为了将光学衍生参数分配给不同的组织类型，有必要构建一个从活检组织获得的光学数据库。本报告展示了可能构成组织鉴别数据库的不同定量和半定量光学标记物，包括：光学指数比、光学氧化还原比、相对胶原密度、光谱分辨荧光寿命参数、双光子荧光成像和二次谐波成像。
