Liu Lihua, Zhao Linlin, Cheng Dandan, Yao Xinyi, Lu Yan
School of Materials Science & Engineering, Tianjin University of Technology, Tianjin 300384, China.
Key Laboratory of Display Materials & Photoelectric Devices, Ministry of Education, Tianjin University of Technology, Tianjin 300384, China.
Polymers (Basel). 2019 Jul 3;11(7):1139. doi: 10.3390/polym11071139.
A boronic acid groups-bearing polythiophene derivate () was designed and synthesized for highly sensitive fluorescence detection of ATP based on a multisite-binding coupled with analyte-induced aggregation strategy. has a polythiophene backbone as fluorophores and two functional side groups, i.e., quaternary ammonium group and boronic acid group, as multibinding sites for ATP. When various structural analogues such as ADP, AMP, and various inorganic phosphates were added into the aqueous solution of , only ATP caused a remarkable fluorescence quenching of about 60-fold accompanied by obvious color changes of solution from yellow to purple. The detection limit is estimated to be 2 nM based on 3/slope. With the advantage of good water solubility, low toxicity, and highly selective response to ATP, was successfully utilized as a probe to real-time assay activity of adenylate kinase (ADK) and map fluorescent imaging of ATP in living cells.
设计并合成了一种含硼酸基团的聚噻吩衍生物（），基于多位点结合与分析物诱导聚集策略用于ATP的高灵敏度荧光检测。具有聚噻吩主链作为荧光团以及两个功能性侧基，即季铵基团和硼酸基团，作为ATP的多结合位点。当将各种结构类似物如ADP、AMP和各种无机磷酸盐添加到的水溶液中时，只有ATP引起约60倍的显著荧光猝灭，并伴随着溶液从黄色到紫色的明显颜色变化。基于3/斜率估计检测限为2 nM。凭借良好的水溶性、低毒性以及对ATP的高选择性响应优势，成功用作探针实时测定腺苷酸激酶（ADK）的活性并绘制活细胞中ATP的荧光成像图。
