适合用于夏氏丝虫属扫描电子显微镜观察的固定剂比较
A comparison of fixatives suitable for scanning electron microscopy of Habronema spp.
作者信息
Krecek R C, Van Niekerk J P, Els H J
机构信息
Department of Parasitology, Faculty of Veterinary Science, University of Pretoria, Private Bag, Onderstepoort.
出版信息
Onderstepoort J Vet Res. 1988 Mar;55(1):63-5.
Members of the genus Habronema (Nematoda: Habronematidae) were preserved in 4 fixatives for examination with scanning electron microscopy (SEM). Tegumental features of the anterior and posterior extremities of these specimens were compared to evaluate the effect of the fixatives: modified Flemming's solution, glutaraldehyde (GA), Karnovsky's fixative and 70% ethanol. Fixatives were assessed on the appearance of the tegument, evidence of any wrinkling, shrinkage or swelling and the degree of extension in the male tail. Seventy per cent ethanol gave the most satisfactory results.
柔线虫属（线虫纲：柔线虫科）的成员保存在4种固定剂中，用于扫描电子显微镜（SEM）检查。将这些标本的前后端体表特征进行比较，以评估固定剂的效果：改良Flemming氏液、戊二醛（GA）、Karnovsky固定剂和70%乙醇。根据体表外观、是否有起皱、收缩或肿胀迹象以及雄性尾部的伸展程度对固定剂进行评估。70%乙醇给出了最令人满意的结果。
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