James S L, Salzman C, Pearce E J
Department of Medicine, George Washington University Medical Center, Washington, DC 20037.
Parasite Immunol. 1988 Jan;10(1):71-83. doi: 10.1111/j.1365-3024.1988.tb00204.x.
Previous studies have shown that many strains of mice develop partial resistance to Schistosoma mansoni as a result of intradermal vaccination with soluble schistosome antigens plus BCG. However, P and BALB/c mice are non-responsive to this intradermal vaccination protocol. In this study, humoral and cellular responses to schistosome antigens in vaccinated P and BALB/c mice were compared to those in protected C57BL/6 mice to identify an immune correlate to resistance in this model. Levels of circulating IgG and IgM antibodies to soluble adult worm antigens, as measured by ELISA, were comparable between strains. Moreover, Western blot analysis revealed no qualitative differences in antibody reactivity, with sera from vaccinated animals of all three strains recognizing the antigen previously identified as Sm-97 (paramyosin). However, vaccinated P and BALB/c mice showed specific defects in cell-mediated immunity to schistosome antigens, including decreased production of macrophage-activating lymphokines and an inability to produce activated macrophage effector cells in vivo at the site of antigen challenge. These observations strengthen our hypothesis that the intradermal vaccine acts through induction of T-cell-mediated immune resistance mechanisms.
先前的研究表明，许多品系的小鼠由于用可溶性血吸虫抗原加卡介苗进行皮内接种而对曼氏血吸虫产生部分抗性。然而，P品系和BALB/c品系小鼠对这种皮内接种方案无反应。在本研究中，将接种疫苗的P品系和BALB/c品系小鼠对血吸虫抗原的体液和细胞反应与受保护的C57BL/6品系小鼠的反应进行比较，以确定该模型中与抗性相关的免疫指标。通过ELISA测定，各品系小鼠针对可溶性成虫抗原的循环IgG和IgM抗体水平相当。此外，蛋白质印迹分析显示抗体反应性没有质的差异，所有三个品系接种疫苗动物的血清均能识别先前鉴定为Sm-97（副肌球蛋白）的抗原。然而，接种疫苗的P品系和BALB/c品系小鼠在针对血吸虫抗原的细胞介导免疫方面表现出特定缺陷，包括巨噬细胞激活淋巴细胞因子产生减少以及在抗原攻击部位无法在体内产生活化的巨噬细胞效应细胞。这些观察结果强化了我们的假设，即皮内疫苗通过诱导T细胞介导的免疫抗性机制发挥作用。
