Prakash Katakam, Sireesha Karanam R
S.N. Vanita Pharmacy Mahavidyalaya, Exhibition grounds, Hyderabad, Andhra Pradesh, India.
Pak J Pharm Sci. 2019 May;32(3):1057-1061.
A simple, sensitive liquid chromatographic method was developed and validated for the simultaneous estimation of sparfloxacin and dexamethasone sodium phosphate in bulk and pharmaceutical formulations. Optimum separation was achieved in less than 10 min using a C18 column (250 mmx4.6 mm i.d, 5μ particle size) by isocratic elution. The mobile phase consisting of a mixture of mixed phosphate buffer (pH 6.8) and acetonitrile (50:50, v/v) was used. Column effluents were monitored at 224nm at a flow rate of 1ml/min. Retention times of sparfloxacin and dexamethasone sodium phosphate were 3.01 and 6.47 min respectively. The linearity of sparfloxacin and dexamethasone sodium phosphate was in the range of 3-18μg/ml and 1-6μg/ml respectively. Developed method was economical because, the time taken and amount of solvent consumed for each analysis was less. The method was validated and was applied to the simultaneous determination of sparfloxacin and dexamethasone sodium phosphate in bulk and pharmaceutical formulations.
建立并验证了一种简单、灵敏的液相色谱法，用于同时测定原料药和药物制剂中司帕沙星和地塞米松磷酸钠的含量。使用C18柱（250 mm×4.6 mm内径，5μm粒径）通过等度洗脱在不到10分钟内实现了最佳分离。流动相由混合磷酸盐缓冲液（pH 6.8）和乙腈（50:50，v/v）的混合物组成。柱流出物在224nm处监测，流速为1ml/min。司帕沙星和地塞米松磷酸钠的保留时间分别为3.01和6.47分钟。司帕沙星和地塞米松磷酸钠的线性范围分别为3-18μg/ml和1-6μg/ml。所开发的方法经济，因为每次分析所需的时间和消耗的溶剂量较少。该方法经过验证，并应用于同时测定原料药和药物制剂中司帕沙星和地塞米松磷酸钠的含量。
