同种异体基质血管成分（SVF）细胞在犬退行性关节疾病中的应用。
The Use of Allogenic Stromal Vascular Fraction (SVF) Cells in Degenerative Joint Disease of the Spine in Dogs.
机构信息
"Kemilew Stem Cells for Animals" Company, Warsaw, Poland.
Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland
出版信息
In Vivo. 2019 Jul-Aug;33(4):1109-1117. doi: 10.21873/invivo.11580.
BACKGROUND/AIM: Stem cells are widely used in regenerative medicine and in clinical practice for the treatment of damaged nerve tissue, myocytes, tendons, and ligaments. The aim of the study was to monitor VEGF levels after the administration of allogenic cellular material (SVF) in the course of treatment of dogs suffering from degenerative joint disease in the spinal region.
MATERIALS AND METHODS
The study was conducted on 10 dogs of both genders, aged between 6 and 13 years in which allogenic stromal vascular fraction of stem cells (SVF) was administered intravenously. The control group was composed of 10 clinically healthy dogs. Before treatment and after 2- and 8-week intervals blood samples were obtained from the study group dogs in order to determine VEGF levels via immunoenzymatic test.
RESULTS
in a few days after the therapy, alleviation of pain symptoms and reduction of lameness were noticed. The VEGF level in 2 weeks after the therapy was significantly elevated (median: 38.77 pg/ml), while in 8 weeks a decrease was observed (median: 18.37 pg/ml). Conlusion: Administration of allogenic stem cells has a positive influence on elevation of the VEGF levels in the blood serum of affected animals as well as their regeneration capacity.
背景/目的：干细胞广泛应用于再生医学和临床实践中，用于治疗受损的神经组织、心肌细胞、肌腱和韧带。本研究的目的是监测接受同种异体细胞物质（SVF）治疗的患有脊柱区域退行性关节病的犬的 VEGF 水平。
材料和方法
本研究在 10 只 6-13 岁的公母犬中进行，这些犬接受了静脉内同种异体基质血管部分细胞（SVF）治疗。对照组由 10 只临床健康犬组成。在治疗前和 2 周和 8 周间隔后，从研究组犬中采集血样，通过免疫酶测试确定 VEGF 水平。
结果
在治疗后几天，疼痛症状减轻，跛行减少。治疗后 2 周 VEGF 水平显著升高（中位数：38.77pg/ml），而 8 周时观察到下降（中位数：18.37pg/ml）。结论：同种异体干细胞的给药对受影响动物的血清 VEGF 水平及其再生能力的提高有积极影响。
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