Bai Yinlei, Chim Chor Sang
a Institute for Immunology and School of Medicine , Tsinghua University , Beijing , People's Republic of China.
b Department of Medicine , The University of Hong Kong , Hong Kong , People's Republic of China.
Hematology. 2019 Dec;24(1):533-537. doi: 10.1080/16078454.2019.1637574.
Buffy coat and ficoll of bone marrow (BM) are viable options for the study of minimal residual disease (MRD) in multiple myeloma (MM). As yet, there is no data about the superiority of either sample types. Herein, we aimed to address this issue.
Forty pairs of ficolled BMs and BM buffy coats of 19 MM patients were studied for MRD by allele-specific oligonucleotide real-time quantitative PCR, with patient-specific primers/probes whenever appropriate.
There were 41 pairs of MRD data for comparison analysis due to one patient with biclonal disease. MRD levels in ficolls and buffy coats were highly concordant ( = 0.936, < 0.0001), with 31 (76%) and seven (17%) pairs being concomitantly MRD-positive or -negative. On the other hand, apart from the 16 pairs being both MRD-negative, or -positive but not quantifiable in ficolls and buffy coats, majority ( = 22, 88%) had higher MRD levels in ficolled BMs than BM buffy coats. Furthermore, in 17 pairs, in which MRD was quantifiable in both, MRD levels in ficolled BMs were 3.1 times those of BM buffy coats (median, 567/10 vs. 184/10, = 0.001).
Taken together, ficolled BM is more sensitive than BM buffy coat for MRD detection in MM, hence should be recommended.
骨髓的血沉棕黄层和聚蔗糖对于多发性骨髓瘤（MM）微小残留病（MRD）的研究是可行的选择。迄今为止，尚无关于这两种样本类型优越性的数据。在此，我们旨在解决这个问题。
对19例MM患者的40对经聚蔗糖处理的骨髓和骨髓血沉棕黄层进行研究，通过等位基因特异性寡核苷酸实时定量PCR检测MRD，必要时使用患者特异性引物/探针。
由于1例患者患有双克隆疾病，因此有41对MRD数据用于比较分析。聚蔗糖和血沉棕黄层中的MRD水平高度一致（r = 0.936，P < 0.0001），其中31对（76%）和7对（17%）同时为MRD阳性或阴性。另一方面，除了16对在聚蔗糖和血沉棕黄层中均为MRD阴性或阳性但不可量化外，大多数（n = 22，88%）经聚蔗糖处理的骨髓中的MRD水平高于骨髓血沉棕黄层。此外，在17对两者MRD均可量化的样本中，经聚蔗糖处理的骨髓中的MRD水平是骨髓血沉棕黄层的3.1倍（中位数，567/10 vs. 184/10，P = 0.001）。
总体而言，经聚蔗糖处理的骨髓在MM的MRD检测中比骨髓血沉棕黄层更敏感，因此应予以推荐。
