Stampfer M J, Jakubowski J A, Faigel D, Vaillancourt R, Deykin D
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115.
Am J Clin Nutr. 1988 Apr;47(4):700-6. doi: 10.1093/ajcn/47.4.700.
A randomized, placebo-controlled double-blind trial was conducted on 20 adults to assess the effect of vitamin E (800 IU/d 727 mg/d for 5 wk) on platelet function, arachidonic acid metabolism, and prostacyclin generation. Platelet aggregation was measured in response to collagen, arachidonic acid, and adenosine diphosphate. Thromboxane B2 was assayed in serum and in the supernatant plasma after platelet aggregation. Platelets were labeled with [3H]arachidonic acid to assess production and release of cyclooxygenase products (MDA, TXB2, and HHT), a lipoxygenase product (12-HETE), and arachidonic acid in response to stimulation by thrombin or collagen. Prostacyclin was measured in plasma and in blood collected from bleeding-time incisions by a sensitive HPLC-RIA procedure. Despite marked increases in plasma and erythrocyte vitamin E levels in the vitamin E group, there were no significant differences between the vitamin E and placebo groups in any of the variables measured.
对20名成年人进行了一项随机、安慰剂对照双盲试验，以评估维生素E（800国际单位/天，727毫克/天，持续5周）对血小板功能、花生四烯酸代谢和前列环素生成的影响。测量血小板对胶原、花生四烯酸和二磷酸腺苷的聚集反应。在血小板聚集后，测定血清和上清血浆中的血栓素B2。用[3H]花生四烯酸标记血小板，以评估凝血酶或胶原刺激后环氧合酶产物（丙二醛、血栓素B2和12-羟基-5,8,10,14-二十碳四烯酸）、脂氧合酶产物（12-羟基二十碳四烯酸）和花生四烯酸的产生和释放。通过灵敏的高效液相色谱-放射免疫分析法测定血浆和从出血时间切口采集的血液中的前列环素。尽管维生素E组血浆和红细胞维生素E水平显著升高，但维生素E组和安慰剂组在任何测量变量上均无显著差异。
