Department of Microsurgery and Orthopedic Trauma, The First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Guangzhou, Guangdong, China (mainland).
Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Hand and Foot Surgery Department, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), Shenzhen, Guangdong, China (mainland).
Med Sci Monit. 2019 Jul 8;25:5044-5053. doi: 10.12659/MSM.917382.
BACKGROUND Recently, ClC-3 chloride channel expression has been noted to be high in some tumors. In chondrosarcoma, which is a malignant tumor with a high incidence in the bone, there has been no previous literature regarding ClC-3 chloride channel expression. Here we evaluated the expression of ClC-3 chloride channel in chondrosarcoma and explored its clinical significance. MATERIAL AND METHODS In this study, 75 chondrosarcoma and 5 normal cartilage tissues were collected. Thereafter, tissue microarray was performed. Immunohistochemistry was also used to observe the level of ClC-3 chloride channel expression between normal and chondrosarcoma tissues. RESULTS Results showed that the expression of ClC-3 chloride channel in the normal chondrocyte was thinner, since it showed distinct differentiation among chondrosarcoma specimens. Interestingly, we noticed that the moderately-differentiated chondrosarcoma (MDC) and the poorly-differentiated chondrosarcoma (PDC) exhibited 94.44% of ClC-3 chloride channel. Besides, the subcellular localization of ClC-3 chloride channel was changed in association with malignant degree changes. The subcellular localization of ClC-3 chloride channel in the MDC and PDC tissue was localized in the cytoplasm and both nucleus and cytoplasm: 83.33% (5 out of 6 cases) and 91.66% (11 out of 12 cases) respectively. On the other hand, we noticed that patient age and gender could have a relation with ClC-3 chloride channel expression; 30- to 60-year-old males showed more expression. CONCLUSIONS These results demonstrated a high frequency of ClC-3 chloride channel overexpression and subcellular localization differences in MDC and PDC tissue, suggesting a specific role of ClC-3 chloride channel in the pathogenesis of chondrosarcoma.
最近，ClC-3 氯离子通道在一些肿瘤中的表达较高。软骨肉瘤是一种在骨骼中发病率较高的恶性肿瘤，之前没有关于 ClC-3 氯离子通道表达的文献。在这里，我们评估了软骨肉瘤中 ClC-3 氯离子通道的表达，并探讨了其临床意义。
本研究收集了 75 例软骨肉瘤和 5 例正常软骨组织，制作组织微阵列，并用免疫组织化学方法观察正常软骨组织和软骨肉瘤组织中 ClC-3 氯离子通道的表达水平。
结果显示，正常软骨细胞中 ClC-3 氯离子通道的表达较薄，因为软骨肉瘤标本之间存在明显的分化。有趣的是，我们注意到中度分化软骨肉瘤（MDC）和低分化软骨肉瘤（PDC）的 ClC-3 氯离子通道表达率均为 94.44%。此外，ClC-3 氯离子通道的亚细胞定位随恶性程度的变化而改变。MDC 和 PDC 组织中 ClC-3 氯离子通道的亚细胞定位定位于细胞质和核质：分别为 83.33%（6 例中有 5 例）和 91.66%（12 例中有 11 例）。另一方面，我们注意到患者年龄和性别可能与 ClC-3 氯离子通道的表达有关；30-60 岁男性的表达更高。
这些结果表明 MDC 和 PDC 组织中 ClC-3 氯离子通道表达频率高且亚细胞定位不同，提示 ClC-3 氯离子通道在软骨肉瘤的发病机制中具有特定作用。
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