a Department of Medical Microbiology, Faculty of Medicine, Medical University of Sofia , Sofia , Bulgaria.
b Department of Medical Chemistry and Biochemistry, Molecular Medicine Center, Faculty of Medicine, Medical University of Sofia , Sofia , Bulgaria.
Infect Dis (Lond). 2019 Sep;51(9):668-675. doi: 10.1080/23744235.2019.1638964. Epub 2019 Jul 8.
The gene, which encodes M protein, is an important epidemiological marker. The aim of this study is to determine the genotypes of Bulgarian clinical streptococccal isolates in 2014-2018 and to evaluate their relationship with virulence genes profiling and disease types. PCR and sequencing were used for genotyping of 182 clinical isolates according to the protocol of the Centre for Disease Control and Prevention. PCR was used to investigate the virulence factors. We identified 15 types and eight clusters. Five main clusters with eight types were predominant: cluster A-C3 () - 24.7%, A-C5 () - 19.2%, E1 () - 11.0%, A-C4 () - 11.0% and E4 () - 20.9%. There were two novel subtypes: and . The investigated strains with genotypes were common in sterile site infections (invasive ones) and types and in skin and mucosal infections. More than 60% of the major cluster A-C3 () members possessed many genes for streptococcal pyrogenic exotoxins that act as super-antigens and bring about potentially higher virulence. : The present study described two novel 3 subtypes. To the best of our knowledge, this study is the first that describe the type spectrum of Bulgarian clinical isolates and associated virulence factors. Monitoring of the pathogenic potential and epidemiology can lead to better knowledge and higher possibility for prevention and eradication of complications of streptococcal infections.
该基因编码 M 蛋白，是重要的流行病学标志物。本研究旨在确定 2014-2018 年保加利亚临床链球菌分离株的基因型，并评估其与毒力基因谱和疾病类型的关系。根据疾病控制和预防中心的方案，通过 PCR 和测序对 182 株临床分离株进行基因分型。PCR 用于研究毒力因子。我们鉴定了 15 种类型和 8 个群。主要有 5 个群 8 种类型占优势：群 A-C3() - 24.7%，A-C5() - 19.2%，E1() - 11.0%，A-C4() - 11.0%和 E4() - 20.9%。有两个新亚型：和。具有基因型的调查菌株常见于无菌部位感染（侵袭性感染）和类型和皮肤及黏膜感染。主要群 A-C3()的 60%以上成员具有许多致热外毒素基因，这些基因作为超抗原，可能具有更高的毒力。结论：本研究描述了两种新的 3 亚型。据我们所知，这是首次描述保加利亚临床分离株的型谱及其相关毒力因子。对致病性潜力和流行病学的监测可以更好地了解和提高预防和消除链球菌感染并发症的可能性。
