伊朗德黑兰住院 5 岁以下急性胃肠炎患儿中人类腺病毒的分子分析。

Molecular analysis of human adenoviruses in hospitalized children <5 years old with acute gastroenteritis in Tehran, Iran.

机构信息

Virology Department, Pasteur Institute of Iran, Tehran, Iran.

Biology Department, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.

出版信息

J Med Virol. 2019 Nov;91(11):1930-1936. doi: 10.1002/jmv.25539. Epub 2019 Jul 18.

DOI:10.1002/jmv.25539

PMID:31283018

Abstract

Human adenoviruses (HAdVs), especially AdV-40 and 41, are common causes of nonbacterial sporadic and outbreak gastroenteritis in children. The present study aimed to describe the frequency and genetic analysis of HAdVs in hospitalized children <5 years old with acute gastroenteritis. A total of 376 stool samples obtained from June 2015 to December 2017 were investigated for the presence of HAdVs by polymerase chain reaction. The HAdV DNA was detected in 16 (4.3%) out of 376 stool samples. Based on the hexon hypervariable region (HVR), B, C, and F HADV species including five types HAdV-1, 2, 3, 6, and 41 were identified, among which enteric AdV species F (EAdV-41) was the most dominant. Moreover, our findings showed the presence of genomic type cluster 1 (GTC1) pattern in Iranian type 41 strains, which was closely similar to the D1 prototype strain (Tak) and D28. In this regard, a recombination was found in AdV-41 strains presenting the hexon sequence that belonged to GTC1, while fiber sequence clustered with GTC2.

摘要

人腺病毒（HAdVs），尤其是 AdV-40 和 41，是儿童非细菌性散发性和爆发性胃肠炎的常见病因。本研究旨在描述急性胃肠炎住院儿童<5 岁的 HAdVs 的频率和遗传分析。2015 年 6 月至 2017 年 12 月共采集 376 份粪便样本，采用聚合酶链反应（PCR）检测 HAdVs 的存在。在 376 份粪便样本中，有 16 份（4.3%）检测到 HAdV DNA。根据六邻体高变区（HVR），B、C 和 F 型 HAdV 包括 5 种 HAdV-1、2、3、6 和 41 种，其中肠道腺病毒 F 型（EAdV-41）最为优势。此外，我们的研究结果表明，伊朗 41 型菌株存在基因组类型簇 1（GTC1）模式，与 D1 原型株（Tak）和 D28 非常相似。在这方面，发现 AdV-41 株存在六邻体序列属于 GTC1 的重组，而纤维序列与 GTC2 聚类。