Department of Internal Medicine, Division of Rheumatology, Hallym University Dongtan Sacred Heart Hospital, Kyunggi, Korea.
Department of Internal Medicine, Division of Rheumatology, Hallym University Sacred Heart Hospital, Anyang, Kyunggi, Korea.
J Orthop Res. 2019 Nov;37(11):2411-2419. doi: 10.1002/jor.24411. Epub 2019 Jul 29.
To investigate the influence of obesity and obligatory bipedal walking on osteoarthritis (OA) development, 26-week-old C57BL/6 mice were divided into two groups and obesity was induced in one group with a 60% fat diet. After 8 weeks, mice from each group were again divided into two groups and obligatory bipedal exercise was induced with a specially designed treadmill in one group, resulting in four experimental groups (control, control bipedal, obese, and obese bipedal). After 8, 10, and 12 weeks of bipedal walking, knee joints were obtained and graded. Surface fibrillation and matrix proteoglycan depletion, began to appear after 8 weeks of exercise in the bipedal groups and progressed as the duration of the exercise increased. At 12 weeks, cartilage loss extending >75% of articular cartilage was observed in none of the control and obese groups, and in 42.8% and 77.7% of control bipedal and obese bipedal animals, respectively. OA grading was significantly higher in the obese bipedal group compared with the control bipedal group. The von Frey fiber test thresholds decreased significantly in the bipedal groups compared with the control and obese groups. This model can be used to study the pathogenesis of human OA and to evaluate its therapeutic agents. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:2411-2419, 2019.
为了研究肥胖和强制性双足行走对骨关节炎（OA）发展的影响，将 26 周龄 C57BL/6 小鼠分为两组，其中一组用 60%脂肪饮食诱导肥胖。8 周后，每组小鼠再次分为两组，其中一组用专门设计的跑步机进行强制性双足运动，共分为四个实验组（对照、对照双足、肥胖、肥胖双足）。在双足行走 8、10 和 12 周后，获取膝关节并进行分级。在双足组运动 8 周后，表面纤维颤动和基质蛋白聚糖耗竭开始出现，并随着运动时间的延长而进展。12 周时，在对照组和肥胖组中，均未观察到软骨损失超过关节软骨 75%的情况，而在对照组双足和肥胖双足动物中，分别有 42.8%和 77.7%出现这种情况。与对照组双足组相比，肥胖双足组的 OA 分级明显更高。与对照组和肥胖组相比，双足组的 von Frey 纤维试验阈值显著降低。该模型可用于研究人类 OA 的发病机制，并评估其治疗药物。©2019 矫形研究学会。由 Wiley Periodicals, Inc. 出版。J Orthop Res 37:2411-2419, 2019。
