Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
PLoS One. 2019 Jul 8;14(7):e0219098. doi: 10.1371/journal.pone.0219098. eCollection 2019.
We applied an in vitro model to evaluate the efficacy of a heparinized 40% ethanol-based lock solution in a wide variety of clinical isolates causing C-RBSI.
A total of 100 clinical strains were collected retrospectively from the blood of patients with C-RBSI. The reduction in biomass and metabolic activity of biofilms was measured using the crystal violet (CV) assay and XTT assay, respectively. Regrowth inhibition (RI) was measured within 24 hours and 72 hours of ethanol lock therapy. Percentage reduction of ≥ 85% in RI was considered to be successful.
Ethanol lock was more effective in reducing metabolic activity than in reducing biomass (83% vs. 50%, respectively). Percentages of RI diminished as regrowth was prolonged (57% for 24 hours and 17% for 72 hours of regrowth). No statistically significant intraspecies differences were found in biofilm reduction or in RI (p>0.05).
The use of heparinized 40% ethanol lock solution for 72 hours significantly reduced biomass and metabolic activity in clinical isolates from patients with C-RBSI. However, as biofilm has an important regrowth rate, 40% ethanol solution was not able to fully eradicate biofilm in vitro.
我们应用体外模型评估肝素化 40%乙醇封管液在引起 C-RBSI 的各种临床分离株中的疗效。
从 C-RBSI 患者的血液中回顾性收集了 100 株临床菌株。使用结晶紫（CV）测定法和 XTT 测定法分别测量生物膜的生物量减少和代谢活性。在乙醇封管治疗 24 小时和 72 小时内测量再生长抑制（RI）。RI 降低率≥85%被认为是成功的。
乙醇封管在降低代谢活性方面比降低生物量更有效（分别为 83%和 50%）。随着再生长的延长，RI 的百分比降低（24 小时再生长时为 57%，72 小时再生长时为 17%）。在生物膜减少或 RI 方面，未发现种内差异有统计学意义（p>0.05）。
肝素化 40%乙醇封管液用于 72 小时可显著降低 C-RBSI 患者临床分离株的生物量和代谢活性。然而，由于生物膜具有重要的再生长率，40%乙醇溶液无法在体外完全清除生物膜。
