斜带石斑鱼（Rachycentron canadum）感染无乳链球菌后头肾和脾脏免疫相关基因的差异表达。
Differential expression of immune-related genes in head kidney and spleen of cobia (Rachycentron canadum) having Streptococcus dysgalactiae infection.
机构信息
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan; International Degree Program of Ornamental Fish Technology and Aquatic Animal Health, International College, National Pingtung University of Science and Technology, Pingtung, Taiwan; Research Center for Fish Vaccines and Diseases, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan; Southern Taiwan Fish Disease Centre, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan; Research Center for Fish Vaccines and Diseases, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.
出版信息
Fish Shellfish Immunol. 2019 Sep;92:842-850. doi: 10.1016/j.fsi.2019.07.009. Epub 2019 Jul 6.
Streptococcus dysgalactiae is a gram-positive bacterium and a harmful aquaculture pathogen. To investigate the immune response against S. dysgalactiae, we performed transcriptome analysis of the head kidney and spleen of cobia (Rachycentron canadum) using RNA-seq. Total RNA was extracted from the head kidney and spleen of cobia, 1 and 2 days after treatment with S. dysgalactiae or control PBS. After RNA purification and cDNA library generation, sequencing was performed using the Illumina HiSeq™ 4000 platform. The filtering and de novo assembling transcripts were annotated using several databases. To identify differentially expressed genes (DEGs) between the S. dysgalactiae and PBS groups, the mapped values of fragments per kilobase of transcripts per million fragments were calculated. After de novo assembly, a total of 106,984 transcripts were detected, with an N50 of 3020 bp. These transcripts were annotated and categorised into a total of 7608 genes based on the KEGG pathway database. DEGs (2-fold difference) were calculated by comparing the S. dysgalactiae and PBS control group gene expression levels at each time point. The DEGs were mainly annotated into signal transduction and immune system categories, based on the KEGG database. The DEGs were significantly enriched in the immune-related pathways - "cytokine-cytokine receptor interaction", "complement and coagulation cascades", and "hematopoietic cell linage". In this study, immune-related genes responding to S. dysgalactiae were detected, and several immune system pathways were categorized. We identified the IL17C-related pathway for inducing the expression of pro-inflammatory cytokine genes (IL-1β, IL-6, and IFNγ). Additionally, neutrophil-related genes (CSF3, CD121, and CD114) were induced in the spleen after S. dysgalactiae infection. It was suggested that these pathways contribute to immune responses against S. dysgalactiae infection. The data revealed in this study may offer improved strategies against S. dysgalactiae infection in cobia.
停乳链球菌是一种革兰氏阳性菌，也是一种有害的水产养殖病原体。为了研究对抗停乳链球菌的免疫反应，我们使用 RNA-seq 对军曹鱼（Rachycentron canadum）的头肾和脾脏进行了转录组分析。从军曹鱼头肾和脾脏中提取总 RNA，在注射停乳链球菌或对照 PBS 后的第 1 天和第 2 天进行处理。在 RNA 纯化和 cDNA 文库生成后，使用 Illumina HiSeq™ 4000 平台进行测序。使用几个数据库对过滤和从头组装的转录本进行注释。为了识别停乳链球菌和 PBS 组之间的差异表达基因（DEG），计算了片段每百万片段的千碱基转录物的映射值。在从头组装后，共检测到 106984 个转录本，N50 为 3020bp。这些转录本根据 KEGG 途径数据库进行注释和分类为总共 7608 个基因。通过比较每个时间点停乳链球菌和 PBS 对照组的基因表达水平，计算 DEG（2 倍差异）。根据 KEGG 数据库，DEG 主要注释为信号转导和免疫系统类别。DEG 在与免疫相关的途径中显著富集 - “细胞因子-细胞因子受体相互作用”、“补体和凝血级联”和“造血细胞谱系”。在这项研究中，检测到了对抗停乳链球菌的免疫相关基因，并对几个免疫系统途径进行了分类。我们确定了与诱导促炎细胞因子基因（IL-1β、IL-6 和 IFNγ）表达相关的 IL17C 途径。此外，停乳链球菌感染后脾脏中诱导了中性粒细胞相关基因（CSF3、CD121 和 CD114）。这表明这些途径有助于对抗停乳链球菌感染的免疫反应。本研究中揭示的数据可能为提高对抗停乳链球菌感染的策略提供依据。
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