日本马传染性泡沫病毒的分离及其血清流行病学调查。

Isolation of an Equine Foamy Virus and Sero-Epidemiology of the Viral Infection in Horses in Japan.

机构信息

Laboratory of Veterinary Virology, Department of Pathobiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, Japan.

Shadai Stallion Station, Abira-cho, Hokkaido 059-1432, Japan.

出版信息

Viruses. 2019 Jul 5;11(7):613. doi: 10.3390/v11070613.

DOI:10.3390/v11070613

PMID:31284407

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6669534/

Abstract

An equine foamy virus (EFV) was isolated for the first time in Japan from peripheral blood mononuclear cells of a broodmare that showed wobbler syndrome after surgery for intestinal volvulus and the isolate was designated as EFVeca_LM. Complete nucleotide sequences of EFVeca_LM were determined. Nucleotide sequence analysis of the long terminal repeat (LTR) region, and genes revealed that EFVeca_LM and the EFV reference strain had 97.2% to 99.1% identities. For a sero-epidemiological survey, indirect immunofluorescent antibody tests were carried out using EFVeca_LM-infected cells as an antigen against 166 sera of horses in five farms collected in 2001 to 2002 and 293 sera of horses in eight farms collected in 2014 to 2016 in Hokkaido, Japan. All of the farms had EFV antibody-positive horses, and average positive rates were 24.6% in sera obtained in 2001 to 2002 and 25.6% in sera obtained in 2014 to 2016 from broodmare farms. The positive rate in a stallion farm (Farm A) in 2002 was 10.7%, and the positive rates in two stallion farms, Farms A and B, in 2015 were 40.9% and 13.3%, respectively. The results suggested that EFV infection is maintained widely in horses in Japan.

摘要

日本首次从患有肠扭转手术后出现摇摆综合征的母马外周血单个核细胞中分离到一株马泡沫病毒（EFV），将分离株命名为 EFVeca_LM。确定了 EFVeca_LM 的完整核苷酸序列。EFVeca_LM 的长末端重复（LTR）区和基因的核苷酸序列分析表明，EFVeca_LM 与 EFV 参考株具有 97.2%至 99.1%的同一性。为了进行血清流行病学调查，使用 EFVeca_LM 感染的细胞作为抗原，对来自北海道五个农场的 2001 年至 2002 年采集的 166 份马血清和 2014 年至 2016 年采集的 293 份马血清进行了间接免疫荧光抗体试验。所有农场都有 EFV 抗体阳性的马，2001 年至 2002 年采集的血清中的平均阳性率为 24.6%，2014 年至 2016 年采集的母马血清中的平均阳性率为 25.6%。2002 年种马场（农场 A）的阳性率为 10.7%，2015 年两个种马场（农场 A 和 B）的阳性率分别为 40.9%和 13.3%。结果表明，EFV 感染在日本马中广泛存在。