Song Jun-Ho, Kim Wook Jin, Cha Ji-Min, Yang Sungyu, Choi Goya, Moon Byeong Cheol
Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju 58245, Korea.
Insects. 2019 Jul 6;10(7):199. doi: 10.3390/insects10070199.
Previous studies have suggested that exuviae can be used for the identification of cicada species, but the precise characteristics that differ among species have not been determined. Thus, we performed the first comparative analyses of the leg morphology, ultrastructure, and mitochondrial DNA sequences of exuviae of four dominant cicada species in Korea, (Motschulsky, 1866), (Walker, 1850), (Fabricius, 1794) and (Fabricius, 1775), the source of Cicadidae Periostracum, a well-known traditional medicine. A morphological analysis revealed that the profemur length, femoral tooth angle, and distance between the intermediate and last tooth of the femoral comb are useful characteristics for identification. We also evaluated the usefulness of the size, degree of reflex, and number of spines on the mid-legs and hind legs as diagnostic features. An ultrastructural study showed that has a unique surface pattern on the legs. The sequences obtained using exuviae were identical to previously obtained sequences for adult tissues. Moreover, in a phylogenetic analysis using sequences, each species formed a monophyletic cluster with high bootstrap support. Accordingly, multiple methodological approaches using exuviae might provide highly reliable identification tools. The integrative data provide useful characteristics for the exuviae-based identification of closely related species and for further taxonomic and systematic studies of Cicadinae.
先前的研究表明，蝉蜕可用于蝉类物种的鉴定，但不同物种之间的确切差异特征尚未确定。因此，我们首次对韩国四种优势蝉类（Motschulsky，1866年）、（Walker，1850年）、（Fabricius，1794年）和（Fabricius，1775年）的蝉蜕腿部形态、超微结构和线粒体DNA序列进行了比较分析，这四种蝉是著名传统药物蝉科蝉蜕的来源。形态学分析表明，前股长度、股齿角度以及股栉中间齿和最后齿之间的距离是有用的鉴定特征。我们还评估了中腿和后腿上的大小、反射程度和刺的数量作为诊断特征的有用性。超微结构研究表明，在腿部具有独特的表面图案。使用蝉蜕获得的序列与先前从成虫组织获得的序列相同。此外，在使用序列进行的系统发育分析中，每个物种都形成了一个具有高自展支持率的单系类群。因此，使用蝉蜕的多种方法可能提供高度可靠的鉴定工具。综合数据为基于蝉蜕的近缘物种鉴定以及叶蝉亚科的进一步分类和系统研究提供了有用的特征。
