果蝇细胞质超氧化物歧化酶编码基因组DNA序列的分离与染色体定位。

Isolation and chromosomal localization of genomic DNA sequences coding for cytoplasmic superoxide dismutase from Drosophila melanogaster.

作者信息

Kirkland K C, Phillips J P

机构信息

Department of Molecular Biology and Genetics, University of Guelph, Ont., Canada.

出版信息

Gene. 1987;61(3):415-9. doi: 10.1016/0378-1119(87)90203-4.

DOI:10.1016/0378-1119(87)90203-4

PMID:3128462

Abstract

A synthetic oligodeoxynucleotide 18-mer probe derived from the amino acid sequence of Drosophila melanogaster cytoplasmic superoxide dismutase (cSOD) was used to screen a D. melanogaster genomic library. One of the positive clones maps by in situ hybridization to position 68A8-9 on the left arm of polytene chromosome 3, the region to which cSOD mutants have previously been mapped genetically. Partial sequence analysis verifies the presence of cSOD-coding sequences in this clone and indicates that the intron structure of the Drosophila cSOD gene differs significantly from its human counterpart.

摘要

一个源自黑腹果蝇细胞质超氧化物歧化酶（cSOD）氨基酸序列的合成18聚体寡脱氧核苷酸探针，被用于筛选黑腹果蝇基因组文库。其中一个阳性克隆通过原位杂交定位到多线染色体3左臂的68A8-9位置，该区域先前已通过遗传学方法定位了cSOD突变体。部分序列分析证实了该克隆中存在cSOD编码序列，并表明果蝇cSOD基因的内含子结构与其人类对应基因有显著差异。