从人乳中分离的鼠李糖乳杆菌无细胞培养上清液的抗肿瘤潜力。
Anti-tumor potential of cell free culture supernatant of Lactobacillus rhamnosus strains isolated from human breast milk.
机构信息
Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China; Department of Food Science and Engineering, College of Chemistry and Chemical Engineering, Shenzhen University, Shenzhen, Guangdong, People's Republic of China.
Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, People's Republic of China.
出版信息
Food Res Int. 2019 Sep;123:286-297. doi: 10.1016/j.foodres.2019.05.002. Epub 2019 May 3.
Lactobacilli rhamnosus has been characterized as a probiotic and plays an important role in human health by stimulating the supplement of nutrients, preventing the colonization of pathogens, and influencing the immune system. This study investigated the anticancer activity of the three Lactobacillus rhamnosus strains SHA111, SHA112, and SHA113 isolated from human breast milk. The cell-free supernatant of a liquid culture of the three strains showed excellent antioxidant activities against DPPH free radicals, superoxide anion radicals, and hydroxyl radicals; furthermore, significant anticancer activity was found on cervix cancer cells (HeLa) via cytotoxicity and induction of apoptosis. RT-qPCR and western blot analysis showed the induction of apoptosis was achieved via the up-regulation of BAD, BAX, Caspase3, Caspase8, Caspase9, and down-regulation of BCL-2 genes in HeLa cells. The results suggest that these strains have potential anticancer capability.
鼠李糖乳杆菌已被鉴定为一种益生菌，通过刺激营养物质的补充、防止病原体的定植和影响免疫系统，在人类健康中发挥着重要作用。本研究调查了从人乳中分离出的三种鼠李糖乳杆菌（SHA111、SHA112 和 SHA113）的抗癌活性。三种菌株液体培养的无细胞上清液对 DPPH 自由基、超氧阴离子自由基和羟自由基具有优异的抗氧化活性；此外，通过细胞毒性和诱导细胞凋亡，发现对宫颈癌细胞（HeLa）具有显著的抗癌活性。RT-qPCR 和 Western blot 分析表明，在 HeLa 细胞中，通过上调 BAD、BAX、Caspase3、Caspase8、Caspase9 基因和下调 BCL-2 基因来诱导细胞凋亡。结果表明，这些菌株具有潜在的抗癌能力。
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