Oil Crops Biotechnology Lab, Agricultural Genetic Engineering Institute, Agricultural Research Center, Giza, Egypt.
Microbiology Lab, Research Park, Faculty of Agriculture, Cairo University, Giza, Egypt.
Sci Rep. 2023 Oct 3;13(1):16601. doi: 10.1038/s41598-023-43752-0.
Lactic acid bacteria (LAB) are of major concern due to their health benefits. Fermented food products comprise variable LAB demonstrating probiotic properties. Discovering and evaluating new probiotics in fermented food products poses a global economic and health importance. Therefore, the present work aimed to investigate and evaluate the probiotic potentials of LAB strains isolated from Egyptian fermented food. In this study, we isolated and functionally characterized 100 bacterial strains isolated from different Egyptian fermented food sources as probiotics. Only four LAB strains amongst the isolated LAB showed probiotic attributes and are considered to be safe for their implementation as feed or dietary supplements. Additionally, they were shown to exert antimicrobial activities against pathogenic bacteria and anticancer effects against the colon cancer cell line Caco-2. The Enterococcus massiliensis IS06 strain was exclusively reported in this study as a probiotic strain with high antimicrobial, antioxidant, and anti-colon cancer activity. Hitherto, few studies have focused on elucidating the impact of probiotic supplementation in vivo. Therefore, in the current study, the safety of the four strains was tested in vivo through the supplementation of rats with potential probiotic strains for 21 days. The results revealed that probiotic bacterial supplementation in rats did not adversely affect the general health of rats. The Lactiplantibacillus plantarum IS07 strain significantly increased the growth performance of rats. Furthermore, the four strains exhibited increased levels of antioxidants such as superoxide dismutase and glutathione in vivo. Consistently, all strains also showed high antioxidant activity of the superoxide dismutase enzyme in vitro. Overall, these findings demonstrated that these isolated potential probiotics harbor desirable characteristics and can be applied widely as feed additives for animals or as dietary supplements for humans to exert their health benefits and combat serious diseases.
乳酸菌（LAB）因其对健康的益处而受到广泛关注。发酵食品包含具有益生菌特性的不同 LAB。发现和评估发酵食品中的新益生菌具有全球经济和健康重要性。因此，本工作旨在研究和评估从埃及发酵食品中分离的 LAB 菌株的益生菌潜力。在这项研究中，我们从不同的埃及发酵食品来源中分离和功能表征了 100 株细菌作为益生菌。在分离的 LAB 中，只有 4 株 LAB 菌株表现出益生菌特性，并且被认为对其作为饲料或膳食补充剂的使用是安全的。此外，它们还表现出对致病菌的抗菌活性和对结肠癌细胞系 Caco-2 的抗癌作用。肠球菌 massiliensis IS06 菌株在本研究中被专门报道为一种具有高抗菌、抗氧化和抗结肠癌活性的益生菌菌株。迄今为止，很少有研究集中在阐明益生菌补充剂在体内的影响。因此，在本研究中，通过在 21 天内用潜在益生菌菌株补充大鼠来测试这 4 株菌株的安全性。结果表明，益生菌细菌补充剂对大鼠的一般健康没有不利影响。植物乳杆菌 IS07 菌株显著提高了大鼠的生长性能。此外，四种菌株在体内表现出抗氧化剂水平的升高，如超氧化物歧化酶和谷胱甘肽。一致地，所有菌株在体外也表现出超氧化物歧化酶酶的高抗氧化活性。总的来说，这些发现表明这些分离的潜在益生菌具有理想的特性，可以广泛应用于动物饲料添加剂或人类膳食补充剂，以发挥其健康益处并对抗严重疾病。
