Diner U E, Kunimoto D, Diener E
J Immunol. 1979 May;122(5):1886-91.
This communication reports on the tolerogenic properties of carboxymethyl cellulose (CMC) as a nonimmunogenic carrier for 2.4 dinitrophenyl (DNP) and the benzylpenicilloyl determinant (BPO). Either normal or primed mice, given an optimal dose of 250 micrograms per animal of DNP CMC, when challenged with an immunogenic form of the hapten as early as 30 min or as late as 21 days thereafter were completely and specifically unresponsive to it. Experimental evidence suggests that this unresponsiveness is not due to suppressor cells. Furthermore, DNP CMC induces tolerance in vivo but fails to do so in vitro under conditions at which other tolerogenic carbohydrate hapten conjugates such as DNP-dextran do. This together with comparative studies of tolerance induction kinetics by DNP CMC and DNP-dextran in vivo led us to conclude that molecular properties other than the epitope density must be attributed to CMC's tolerogenic potential. CMC may also be used as a tolerogenic carrier for BPO with respect to IgE antibody production. Thus, normal or primed mice injected with the BPO CMC conjugate were found specifically unresponsive to a challenge with an immunogenic form of penicillin.
本通讯报道了羧甲基纤维素（CMC）作为2,4-二硝基苯基（DNP）和苄青霉素酰决定簇（BPO）的非免疫原性载体的致耐受性特性。无论是正常小鼠还是致敏小鼠，每只动物给予250微克的最佳剂量DNP CMC，在之后30分钟或长达21天用半抗原的免疫原形式进行攻击时，对其完全无特异性反应。实验证据表明这种无反应性并非由于抑制细胞。此外，DNP CMC在体内诱导耐受性，但在其他致耐受性碳水化合物半抗原缀合物如DNP-葡聚糖能诱导耐受性的条件下，在体外却不能。这与DNP CMC和DNP-葡聚糖在体内诱导耐受性动力学的比较研究一起，使我们得出结论，除表位密度外的分子特性必定归因于CMC的致耐受性潜力。就IgE抗体产生而言，CMC也可用作BPO的致耐受性载体。因此，发现注射了BPO CMC缀合物的正常或致敏小鼠对青霉素免疫原形式的攻击无特异性反应。
