Institute of Burn Research, State Key Laboratory of Trauma, Burns and Combined Injury, Southwest Hospital, Army Military Medical University, Chongqing, China.
State Key Laboratory of Trauma, Burns and Combined Injury, Plastic Surgery Department, Southwest Hospital, Army Military Medical University, Chongqing, China.
Methods Mol Biol. 2020;2109:219-224. doi: 10.1007/7651_2019_248.
Reepithelialization of wounds involves several keratinocyte functions: proliferation, migration, and differentiation. The key step is keratinocyte migration, an essential aspect for understanding chronic non-healing wounds. Scratch wounding assay and cell motility assay are used to evaluate keratinocyte migration in vitro. The ability of keratinocyte migration is measured by the dynamic change of the wound closure area over time under a living-cell workstation. NIH ImageJ software is used to quantify the motility ability of single cell. The following protocols offer a general guideline for keratinocyte migration analysis.
增殖、迁移和分化。关键步骤是角质形成细胞迁移，这是理解慢性难愈性伤口的一个重要方面。划痕实验和细胞迁移实验用于评估体外角质形成细胞迁移。在活细胞工作站下，通过测量随时间推移伤口闭合面积的动态变化来评估角质形成细胞迁移的能力。NIH ImageJ 软件用于量化单细胞的迁移能力。以下方案为角质形成细胞迁移分析提供了一般指南。
