Molecular Biology Division, Biomedical Center, Ludwig-Maximilians-University, Munich, Germany.
Graduate School of Quantitative Biosciences Munich, Ludwig-Maximilians-University, Munich, Germany.
EMBO Rep. 2019 Aug;20(8):e48138. doi: 10.15252/embr.201948138. Epub 2019 Jul 9.
In Drosophila melanogaster males, X-chromosome monosomy is compensated by chromosome-wide transcription activation. We found that complete dosage compensation during embryogenesis takes surprisingly long and is incomplete even after 10 h of development. Although the activating dosage compensation complex (DCC) associates with the X-chromosome and MOF acetylates histone H4 early, many genes are not compensated. Acetylation levels on gene bodies continue to increase for several hours after gastrulation in parallel with progressive compensation. Constitutive genes are compensated earlier than developmental genes. Remarkably, later compensation correlates with longer distances to DCC binding sites. This time-space relationship suggests that DCC action on target genes requires maturation of the active chromosome compartment.
在黑腹果蝇雄性中，X 染色体单体通过染色体-wide 转录激活得到补偿。我们发现，胚胎发生过程中的完全剂量补偿需要很长时间，即使在发育 10 小时后也不完全。尽管激活的剂量补偿复合物（DCC）与 X 染色体结合，并且 MOF 早期乙酰化组蛋白 H4，但许多基因并未得到补偿。在原肠胚形成后几个小时内，基因主体上的乙酰化水平继续增加，与逐渐补偿平行。组成性基因比发育基因更早得到补偿。值得注意的是，后期补偿与 DCC 结合位点的距离较长相关。这种时-空关系表明，DCC 对靶基因的作用需要活性染色体区室的成熟。
