School of Chemistry and Chemical Engineering, and Institute of Molecular Medicine, Renji Hospital, School of Medicine , Shanghai Jiao Tong University , Shanghai 200240 , China.
Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Synchrotron Radiation Facility , Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800 , China.
J Am Chem Soc. 2019 Jul 31;141(30):11938-11946. doi: 10.1021/jacs.9b03498. Epub 2019 Jul 18.
The rapidly increasing need for systems biology stimulates the development of supermultiplex (SM) methods for simultaneously labeling multiple biomolecules/cells with distinct colors. Here we report the development of DNA-engineered fractal nanoplasmonic labels with ultrahigh brightness and photostability for SM imaging in single cells. These color-resolvable nanoplasmonic labels have a uniform size of ∼50 nm with an inner hollow gap of ∼1 nm. The outer shell morphology is highly tunable with the tip aspect ratio covering the range of δ = 0.29-1.66, which supports SM plasmonic imaging exceeding the conventional fluorescence multiplexing limit. We demonstrate the use of these SM labels for quantitative imaging of receptor-mediated endocytosis and intracellular transport of multiple protein-NP structures in a single cell in real time. This SM-plasmonic method sheds light on elucidating complex interactions among protein-NPs in nanotoxicology and facilitates the development of novel nanomedicines for diagnosis and therapy.
对系统生物学日益增长的需求刺激了超多重（SM）方法的发展，这种方法可将多个具有不同颜色的生物分子/细胞同时进行标记。在这里，我们报告了具有超高亮度和光稳定性的 DNA 工程化分形纳米等离子体标签的开发，可用于单细胞中的 SM 成像。这些可分辨颜色的纳米等离子体标签具有约 50nm 的均匀尺寸，内有空隙约 1nm。外壳形态具有高度可调性，尖端纵横比覆盖范围为 δ = 0.29-1.66，支持超过传统荧光多重化限制的 SM 等离子体成像。我们证明了这些 SM 标签可用于实时定量成像单个细胞中受体介导的内吞作用和多种蛋白-NP 结构的细胞内运输。这种 SM-等离子体方法有助于阐明纳米毒理学中蛋白-NP 之间的复杂相互作用，并促进新型诊断和治疗用纳米药物的开发。
