The RNA Institute, University at Albany, State University of New York, NY 12222, USA.
Department of Biological Sciences, University at Albany, State University of New York, NY 12222, USA.
Nucleic Acids Res. 2019 Nov 18;47(20):10489-10505. doi: 10.1093/nar/gkz580.
MicroRNAs are involved in the crucial processes of development and diseases and have emerged as a new class of biomarkers. The field of DNA nanotechnology has shown great promise in the creation of novel microRNA biosensors that have utility in lab-based biosensing and potential for disease diagnostics. In this Survey and Summary, we explore and review DNA nanotechnology approaches for microRNA detection, surveying the literature for microRNA detection in three main areas of DNA nanostructures: DNA tetrahedra, DNA origami, and DNA devices and motifs. We take a critical look at the reviewed approaches, advantages and disadvantages of these methods in general, and a critical comparison of specific approaches. We conclude with a brief outlook on the future of DNA nanotechnology in biosensing for microRNA and beyond.
MicroRNAs 参与了发育和疾病的关键过程，已成为一类新的生物标志物。DNA 纳米技术领域在新型 microRNA 生物传感器的创建方面显示出巨大的潜力，这些传感器在基于实验室的生物传感方面具有实用性，并有可能用于疾病诊断。在本调查和综述中，我们探索和回顾了用于 microRNA 检测的 DNA 纳米技术方法，调查了 DNA 四面体、DNA 折纸和 DNA 器件和图案这三个主要 DNA 结构领域中的 microRNA 检测文献。我们对所审查的方法进行了批判性的分析，概括了这些方法的优缺点，并对具体方法进行了批判性比较。最后，我们简要展望了 DNA 纳米技术在 microRNA 及其他生物传感方面的未来。
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