Lee Ju Hun, Kim Hyeong Ryeol, Lee Ja Hyun, Lee Soo Kweon, Chun Youngsang, Han Sung Ok, Yoo Hah Young, Park Chulhwan, Kim Seung Wook
Department of Chemical and Biological Engineering, Korea University, 145, Anam-Ro, Seongbuk-Gu, Seoul 02841, Korea.
Department of Food Science and Engineering, Dongyang Mirae University, 445, Gyeongin-ro, Guro-gu, Seoul 08221, Korea.
Polymers (Basel). 2019 Jul 8;11(7):1162. doi: 10.3390/polym11071162.
Conductive biopolymers, an important class of functional materials, have received attention in various fields because of their unique electrical, optical, and physical properties. In this study, the polymerization of heme into hemozoin was carried out in an in vitro system by the newly developed heme polymerase (histidine-rich protein 2 (HRP-II)). The HRP-II was produced by recombinant BL21 from the gene. To improve the hemozoin production, the reaction conditions on the polymerization were investigated and the maximum production was achieved after about 790 μM at 34 °C with 200 rpm for 24 h. As a result, the production was improved about two-fold according to the stepwise optimization in an in vitro system. The produced hemozoin was qualitatively analyzed using the Fourier transform infrared (FTIR) spectroscopy, energy dispersive X-ray spectroscopy (EDS), and scanning electron microscopy (SEM). Finally, it was confirmed that the enzymatically polymerized hemozoin had similar physical properties to chemically synthesized hemozoin. These results could represent a significant potential for nano-biotechnology applications, and also provide guidance in research related to hemozoin utilization.
导电生物聚合物是一类重要的功能材料，因其独特的电学、光学和物理性质而在各个领域受到关注。在本研究中，通过新开发的血红素聚合酶（富含组氨酸的蛋白2，HRP-II）在体外系统中进行血红素向疟原虫色素的聚合反应。HRP-II由重组BL21从该基因产生。为提高疟原虫色素的产量，研究了聚合反应条件，在34℃、200 rpm下反应24小时，约790μM时达到最大产量。结果，根据体外系统中的逐步优化，产量提高了约两倍。使用傅里叶变换红外光谱（FTIR）、能量色散X射线光谱（EDS）和扫描电子显微镜（SEM）对产生的疟原虫色素进行了定性分析。最后，证实酶促聚合的疟原虫色素具有与化学合成的疟原虫色素相似的物理性质。这些结果可能代表了纳米生物技术应用的巨大潜力，也为与疟原虫色素利用相关的研究提供了指导。
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