Rahman Md Saifur, Choi Yoon Seok, Kim Young Kyun, Park Chulhwan, Yoo Jin Cheol
Department of Pharmacy, College of Pharmacy, Chosun University, 309, Pilmun-daero, Dong-gu, Gwangju 61452, Korea.
Department of Chemical Engineering, Kwangwoon University, 20, Kwangwoon-ro, Nowon-gu, Seoul 01897, Korea.
Polymers (Basel). 2019 Sep 19;11(9):1525. doi: 10.3390/polym11091525.
Polygalacturonase (EC. 3.2.1.15) is an enzyme that hydrolyzes the alpha-1,4 glycosidic bonds between galacturonic acid. In this study, an alkaline polygalacturonase producer CBS32 was isolated from kimchi (conventional Korean fermented food). The 16S rRNA sequence analysis of the isolated strain revealed that it was 99.92% identical to KJ 16LBMN01000156. The polygalacturonase from CBS32 was named PN32, and the purified PN32 showed a 16.8% yield and a 33-fold purity compared to the crude broth. The molecular mass, 110 kDa, was determined by SDS-PAGE, and the active band was confirmed by zymography analysis. The N-terminal amino acid sequence residues of PN32 were determined to be Gly-Val-Lys-Glu-Val-X-Gln-Thr-Phe. In the sequence comparison, PN32 was suggested as a novel polygalacturonase, since the sequence was not matched with the previous reports. In an application study, enzymatic depolymerization of ramie was performed for fiber degumming, and the result showed that the PN32 had a 28% higher depolymerization compared to the commercial pectinase. Overall, based on the results, PN32 has high potential for industrial applications.
聚半乳糖醛酸酶（EC. 3.2.1.15）是一种能够水解半乳糖醛酸之间α-1,4糖苷键的酶。在本研究中，从泡菜（传统韩国发酵食品）中分离出了一株碱性聚半乳糖醛酸酶产生菌CBS32。对分离菌株进行的16S rRNA序列分析表明，它与KJ 16LBMN01000156的序列相似度为99.92%。来自CBS32的聚半乳糖醛酸酶被命名为PN32，与粗发酵液相比，纯化后的PN32产率为16.8%，纯度提高了33倍。通过SDS-PAGE测定其分子量为110 kDa，并通过酶谱分析确认了活性条带。PN32的N端氨基酸序列残基确定为Gly-Val-Lys-Glu-Val-X-Gln-Thr-Phe。在序列比较中，PN32被认为是一种新型聚半乳糖醛酸酶，因为其序列与先前的报道不匹配。在一项应用研究中，进行了苎麻的酶促解聚以进行纤维脱胶，结果表明PN32的解聚率比市售果胶酶高28%。总体而言，基于这些结果，PN32具有很高的工业应用潜力。
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