在无DNA合成情况下细胞溶解性T淋巴细胞的初始产生。
Primary generation of cytolytic T lymphocytes in the absence of DNA synthesis.
作者信息
MacDonald H R, Less R K
出版信息
J Exp Med. 1979 Jul 1;150(1):196-201. doi: 10.1084/jem.150.1.196.
The requirement for DNA synthesis during the primary differentiation of cytolytic T lymphocytes (CTL) had been investigated. CTL were induced polyclonally in vitro by stimulation of normal C57BL/6 spleen cells with concanavalin A (Con A)and their cytolytic activity was tested against 51Cr-labeled target cells in the presence of Bacto Phytohemagglutinin M. With this system, CTL activity could first be detected 48 h after exposure of spleen cells to Con A. Addition of cytosine arabinoside at concentrations sufficient to reduce DNA synthesis by 95-98% in Con A-stimulated cultures did not significantly inhibit the generation of cytolytic activity on a cell-to-cell basis. These results demonstrate that derepression of the genetic information required for the expression of CTL function can occur in the absence of detectable DNA synthesis.
对细胞毒性T淋巴细胞（CTL）初次分化过程中DNA合成的需求进行了研究。通过用伴刀豆球蛋白A（Con A）刺激正常C57BL/6脾细胞，在体外多克隆诱导CTL，并在存在细菌植物血凝素M的情况下，针对51Cr标记的靶细胞测试其细胞溶解活性。在这个系统中，脾细胞暴露于Con A后48小时首次检测到CTL活性。在Con A刺激的培养物中添加足以使DNA合成减少95-98%的阿糖胞苷，在细胞对细胞的基础上并没有显著抑制细胞溶解活性的产生。这些结果表明，在没有可检测到的DNA合成的情况下，CTL功能表达所需的遗传信息的去抑制可以发生。
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