3-脱氧葡萄糖酮通过下调 STC-1 细胞中的 SGLT1 表达来降低低血糖水平下胰高血糖素样肽-1 的分泌。
3-Deoxyglucosone reduces glucagon-like peptide-1 secretion at low glucose levels through down-regulation of SGLT1 expression in STC-1 cells.
机构信息
Suzhou Academy of Wumen Chinese Medicine, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, China.
出版信息
Arch Physiol Biochem. 2021 Aug;127(4):311-317. doi: 10.1080/13813455.2019.1638413. Epub 2019 Jul 10.
CONTEXT
Sodium glucose co-transporter 1 (SGLT1) triggers low glucose-induced glucagon-like peptide-1 (GLP-1) secretion. We reported that a two-week administration of 3-deoxyglucosone (3DG), an independent factor associated with the development of pre-diabetes, reduces basal GLP-1 secretion in rats.
OBJECTIVE
This study investigated the effects of 3DG on GLP-1 secretion and SGLT1 pathway under low-glucose conditions in STC-1 cells.
METHODS
STC-1 cells were incubated with phloridzin or 3DG at 5.6 mM glucose. SGLT1 expression (by western blotting), GLP-1 and cyclic adenosine monophosphate (cAMP) levels (by ELISA), and intracellular Ca concentration (by Fluo-3/AM) were measured.
RESULTS
Phloridzin inhibited GLP-1 secretion. SGLT1 protein expression in STC-1 cells cultured in 5.6 mM glucose is higher than that in 25 mM glucose. Exposure to 3DG for 6 h reduced GLP-1 secretion, SGLT1 protein expression, and intracellular concentrations of cAMP and Ca.
CONCLUSIONS
3DG reduces low glucose-induced GLP-1 secretion in part through reduction of SGLT1 expression.
背景
钠-葡萄糖共转运蛋白 1（SGLT1）触发低血糖诱导的胰高血糖素样肽-1（GLP-1）分泌。我们曾报道，为期两周的 3-脱氧葡萄糖（3DG）处理，一种与糖尿病前期发展相关的独立因素，可降低大鼠基础 GLP-1 分泌。
目的
本研究旨在探讨 3DG 对 STC-1 细胞在低血糖条件下 GLP-1 分泌和 SGLT1 途径的影响。
方法
用 phloridzin 或 3DG 在 5.6mmol/L 葡萄糖下孵育 STC-1 细胞。通过 Western blot 测定 SGLT1 表达，通过 ELISA 测定 GLP-1 和环磷酸腺苷（cAMP）水平，通过 Fluo-3/AM 测定细胞内 Ca2+浓度。
结果
phloridzin 抑制 GLP-1 分泌。在 5.6mmol/L 葡萄糖中培养的 STC-1 细胞中 SGLT1 蛋白表达高于 25mmol/L 葡萄糖。暴露于 3DG 6 小时可减少 GLP-1 分泌、SGLT1 蛋白表达以及细胞内 cAMP 和 Ca2+浓度。
结论
3DG 通过降低 SGLT1 表达部分减少低血糖诱导的 GLP-1 分泌。
Zotero 插件