Wu Yanyan, Cai Qiuxing, Li Laihao, Wang Yueqi, Yang Xianqing
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Key Lab of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs of the People's Republic of China.
Guangxi College and Universities Key Laboratory Development and High-value Utilization of Beibu Gulf Seafood Resources, College of Food Engineering, Beibu Gulf University.
J Oleo Sci. 2019 Aug 1;68(8):769-779. doi: 10.5650/jos.ess19032. Epub 2019 Jul 10.
In order to reveal changes in fatty acids and triglycerides during the pickling process of white-fleshed and dark-fleshed fish with high-fat, to compare the changes of triacylglycerols (TAGs) and fatty acids (FAs) in round scad (Decapterus maruadsi, dark-fleshed) and hairtail (Trichiurus lepturus, white-fleshed) during salt-dried processing, ESI-MS/MS and GC-MS techniques were used to quantify. Lipid oxidation was evaluated via peroxide values (POVs), and thiobarbituric reactive substances (TBARS). A total of 31 and 27 FAs, 45 and 44 TAGs were quantified in round scad and hairtail, respectively. DHA (C22:6n3), palmitic acid (C16:0), stearic acid (C18:0), and oleic acid (C18:1n9) were the main FAs in round scad. POO (16:0/18:1/18:1), PPO (16:0/16:0/18:1), POD (16:0/18:1/22:6), and PPaO (16:0/16:1/18:1) were dominant TAGs in both species. Salt-dried processing significantly affected (p < 0.001) 7/5 FAs and 24/29 TAGs in round scad/hairtail. MUFAs changed significantly (p < 0.05) in dark-fleshed round scad; only SFAs and PUFAs changed in white-fleshed hairtail. Both species exhibited near-identical TAG compositions with different variation trends. More significant changes were observed in FAs at the half-dried stages and in TAGs (p < 0.05) at the salted stage. This coincided with the changing stages of POV and TBARS values that also increased significantly (p < 0.05) at the salted stages but peaked at the half-dried stages of both species.
为了揭示高脂肪白肉和红肉鱼类腌制过程中脂肪酸和甘油三酯的变化,比较圆腹鲱(Decapterus maruadsi,红肉)和带鱼(Trichiurus lepturus,白肉)在盐干加工过程中三酰甘油(TAGs)和脂肪酸(FAs)的变化,采用电喷雾串联质谱(ESI-MS/MS)和气相色谱-质谱联用(GC-MS)技术进行定量分析。通过过氧化值(POVs)和硫代巴比妥酸反应物(TBARS)评估脂质氧化。在圆腹鲱和带鱼中分别定量了31种和27种脂肪酸、45种和44种甘油三酯。二十二碳六烯酸(C22:6n3)、棕榈酸(C16:0)、硬脂酸(C18:0)和油酸(C18:1n9)是圆腹鲱中的主要脂肪酸。1-棕榈酰-2,3-二油酰甘油(POO,16:0/18:1/18:1)、1,2-二棕榈酰-3-油酰甘油(PPO,16:0/16:0/18:1)、1-棕榈酰-2-油酰-3-二十二碳六烯酰甘油(POD,16:0/18:1/22:6)和1-棕榈酰-2-反式-16:1-3-油酰甘油(PPaO,16:0/16:1/18:1)是两种鱼中的主要甘油三酯。盐干加工对圆腹鲱/带鱼中的7/5种脂肪酸和24/29种甘油三酯有显著影响(p < 0.001)。红肉的圆腹鲱中多不饱和脂肪酸(MUFAs)有显著变化(p < 0.05);白肉的带鱼中只有饱和脂肪酸(SFAs)和多不饱和脂肪酸(PUFAs)发生了变化。两种鱼的甘油三酯组成相近,但变化趋势不同。在半干阶段,脂肪酸的变化更为显著,在腌制阶段,甘油三酯有显著变化(p < 0.05)。这与过氧化值(POV)和硫代巴比妥酸反应物(TBARS)值的变化阶段一致,在腌制阶段,它们也显著增加(p < 0.05),但在两种鱼的半干阶段达到峰值。