Department of Obstetrics, Jining No. 1 People's Hospital, Affiliated Jining No. 1 People's Hospital of Jining Medical University, Jining Medical University, Jining, Shandong, China (mainland).
Department of Obstetrics, Yantaishan Hospital, Yantai, Shandong, China (mainland).
Med Sci Monit. 2019 Jul 11;25:5137-5142. doi: 10.12659/MSM.914930.
BACKGROUND This study aimed to investigate the association between the rs2975760 and rs3792267 single nucleotide polymorphisms (SNPs) of the calpain 10 (CAPN10) gene and gestational diabetes mellitus. MATERIAL AND METHODS The study included 138 patients with gestational diabetes mellitus and 152 healthy pregnant women. Venous blood was separated, and the DNA was extracted. The rs2975760 and rs3792267SNP polymorphisms of CAPN10 were detected using polymerase chain reaction (PCR). The frequencies of different genotypes in patients with gestational diabetes mellitus and healthy pregnant women were determined, and the relationship between different SNP genotypes and the risk of gestational diabetes mellitus was analyzed. RESULTS There were no significant differences in the frequencies of the TT, CT and CC genotypes of rs2975760 and the frequencies of the GG, AG and AA genotypes of rs3792267 between the women with gestational diabetes and the controls. Expression of rs2975760 and rs3792267 were not associated with the risk of gestational diabetes in the dominant model, recessive model, and additive model. However, grade B and grade D diabetes in the CC and TC genotypes of rs2975760 were significantly different from those in the TT genotype (P<0.05). Grade B and grade D diabetes in the AA and AG genotypes of rs3792267 were significantly different compared with those in the GG genotype (P<0.05), and allele A was significantly increased compared with allele G (P<0.05). CONCLUSIONS The rs2975760 and rs3792267 SNP polymorphisms of CAPN10 showed no significant association with the incidence of gestational diabetes mellitus and only a mild association with the severity.
背景 本研究旨在探讨钙蛋白酶 10（CAPN10）基因 rs2975760 和 rs3792267 单核苷酸多态性（SNP）与妊娠期糖尿病之间的关系。
材料与方法 本研究纳入了 138 例妊娠期糖尿病患者和 152 例健康孕妇。分离静脉血并提取 DNA。采用聚合酶链反应（PCR）检测 CAPN10 中 rs2975760 和 rs3792267SNP 多态性。确定妊娠期糖尿病患者和健康孕妇中不同基因型的频率，并分析不同 SNP 基因型与妊娠期糖尿病的风险关系。
结果 rs2975760 的 TT、CT 和 CC 基因型以及 rs3792267 的 GG、AG 和 AA 基因型在妊娠期糖尿病患者和对照组之间的频率无显著差异。在显性模型、隐性模型和加性模型中，rs2975760 和 rs3792267 的表达与妊娠期糖尿病的发病风险无关。然而，在 rs2975760 的 CC 和 TC 基因型中，B 级和 D 级糖尿病与 TT 基因型显著不同（P<0.05）。在 rs3792267 的 AA 和 AG 基因型中，B 级和 D 级糖尿病与 GG 基因型显著不同（P<0.05），且等位基因 A 明显高于等位基因 G（P<0.05）。
结论 CAPN10 的 rs2975760 和 rs3792267SNP 多态性与妊娠期糖尿病的发病率无显著关联，仅与严重程度有轻微关联。
