Nollen P M, Pyne J L
J Parasitol. 1979 Feb;65(1):35-7.
Upon in vitro exposure to 3H-adenosine, spermatogonial cells in adult Megalodiscus temperatus were readily labeled on autoradiograms. At least 20 days were required for these cells to produce labeled sperm, the longest time reported so far for digenetic trematodes. Labelled worms transplanted to hosts for 24--31 days showed both self- and cross-insemination in multiple infections. When alone, half of the transplanted adults self-inseminated.
成年温和巨盘吸虫的精原细胞在体外暴露于3H-腺苷后，在放射自显影片上很容易被标记。这些细胞产生标记精子至少需要20天，这是迄今为止报道的双殖吸虫最长的时间。移植到宿主体内24至31天的标记虫在多重感染中表现出自体受精和异体受精。单独饲养时，一半的移植成虫进行了自体受精。
