两种跳过细胞裂解和蛋白质提取步骤的方法用于通过基质辅助激光解吸/电离飞行时间质谱法从血培养物中鉴定细菌的比较
Comparison of two methods skipping cell lysis and protein extraction for identification of bacteria from blood cultures by matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry.
作者信息
Torres I, Giménez E, Huntley D, Martínez M, Colomina J, Navarro D
机构信息
David Navarro, Microbiology Service, Hospital Clínico Universitario, and Department of Microbiology, School of Medicine. Av. Blasco Ibáñez 17, 46010 Valencia, Spain.
出版信息
Rev Esp Quimioter. 2019 Aug;32(4):365-369. Epub 2019 Jul 10.
OBJECTIVE
Matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS) is widely used for fast identification of bacteria from blood cultures (BC). We compared the performance of two procedures, one including a pre-enrichment step in brain heart infusion and the other a direct method using vacutainer separator gel tubes (DI), for identification of bacteria from blood cultures by MALDI-TOF MS.
METHODS
We first prepared a training set of 20 simulated bacteremia specimens, including 10 Gram-negative and 10 Gram-positive species. A total of 145 non-consecutive BCs flagged as positive (68 Gram-negative rods, and 77 Gram-positive cocci) were prospectively analyzed (validation set).
RESULTS
A total of 82% and 49% of isolates were correctly identified to the species level by the respective methods.
CONCLUSIONS
The pre-enrichment method outperformed the DI method for identification of virtually all bacterial species included in the panels.
目的
基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）被广泛用于从血培养物（BC）中快速鉴定细菌。我们比较了两种方法的性能，一种方法是在脑心浸液中进行预富集步骤，另一种是使用真空管分离胶管的直接方法（DI），用于通过MALDI-TOF MS从血培养物中鉴定细菌。
方法
我们首先制备了一组包含20个模拟菌血症标本的训练集，其中包括10种革兰氏阴性菌和10种革兰氏阳性菌。前瞻性分析了总共145份标记为阳性的非连续血培养物（验证集）（68株革兰氏阴性杆菌和77株革兰氏阳性球菌）。
结果
通过各自的方法，分别有82%和49%的分离株被正确鉴定到种水平。
结论
对于鉴定面板中几乎所有细菌种类，预富集方法优于DI方法。
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