Echtenacher B, Hederer R, Krammer P H
Institute of Immunology and Genetics, German Cancer Research Center, Heidelberg.
Immunobiology. 1987 Dec;176(1-2):96-107. doi: 10.1016/s0171-2985(87)80103-1.
This paper describes the biological effect of monoclonal antibodies to murine IFN-gamma produced by in vitro immunization with only several nanograms of rIFN-gamma. Four mAbs binding to rIFN-gamma were selected. mAb U7 was characterized in detail and shown to bind specifically to rIFN-gamma in a Western blot and to specifically inhibit the antiviral effect of rIFN-gamma and natural IFN-gamma. The activities of IFN-alpha, beta and IL2 were not affected. The M phi activating effect of both rIFN-gamma and natural IFN-gamma was also inhibited by mAb U7. Thus, we showed that it is possible to produce specific mAbs with very small amounts of cytokines by in vitro immunization.
本文描述了仅用几纳克重组干扰素-γ（rIFN-γ）进行体外免疫产生的抗小鼠干扰素-γ单克隆抗体的生物学效应。筛选出了4种与rIFN-γ结合的单克隆抗体。对单克隆抗体U7进行了详细表征，结果显示其在蛋白质免疫印迹中能特异性结合rIFN-γ，并能特异性抑制rIFN-γ和天然干扰素-γ的抗病毒效应。干扰素-α、β及白细胞介素2的活性未受影响。单克隆抗体U7也抑制了rIFN-γ和天然干扰素-γ对巨噬细胞的激活作用。因此，我们证明了通过体外免疫用极少量的细胞因子就能产生特异性单克隆抗体。
