Mensah-Brown Henrietta E, Aspeling-Jones Harvey, Delimini Rupert K, Asante Kwaku Poku, Amlabu Emmanuel, Bah Saikou Y, Beeson James G, Wright Gavin J, Conway David J, Awandare Gordon A
West African Centre for Cell Biology of Infectious Pathogens, University of Ghana, Legon, Accra, Ghana.
Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon, Accra, Ghana.
Open Forum Infect Dis. 2019 May 28;6(7):ofz254. doi: 10.1093/ofid/ofz254. eCollection 2019 Jul.
uses a repertoire of merozoite-stage proteins for invasion of erythrocytes. Antibodies against some of these proteins halt the replication cycle of the parasite by preventing erythrocyte invasion and are implicated as contributors to protective immunity against malaria.
We assayed antibody reactivity against a panel of 9 recombinant antigens based on erythrocyte-binding antigen (EBA) and reticulocyte-like homolog (Rh) proteins in plasma from children with malaria and healthy adults residing in 3 endemic areas in Ghana using enzyme-linked immunosorbent assay. Purified immunoglobulin (Ig)G from adult plasma samples was also tested for invasion inhibition against 7 different culture lines, including clinical isolates.
Antibodies against the antigens increased in an age-dependent manner in children. Breadth of reactivity to the different antigens was strongly associated with in vitro parasite growth inhibitory activity of IgG purified from the adults. The strongest predictors of breadth of antibody reactivity were age and transmission intensity, and a combination of reactivities to Rh2, Rh4, and Rh5 correlated strongly with invasion inhibition.
Growth inhibitory activity was significantly associated with breadth of antibody reactivity to merozoite antigens, encouraging the prospect of a multicomponent blood-stage vaccine.
疟原虫裂殖子阶段利用一系列蛋白质侵入红细胞。针对其中一些蛋白质的抗体可通过阻止红细胞入侵来阻断寄生虫的复制周期，并被认为是疟疾保护性免疫的促成因素。
我们使用酶联免疫吸附测定法，检测了来自加纳3个疟疾流行地区的疟疾患儿和健康成年人血浆中，针对一组基于红细胞结合抗原（EBA）和网织红细胞样同源物（Rh）蛋白的9种重组抗原的抗体反应性。还检测了来自成人血浆样本的纯化免疫球蛋白（Ig）G对7种不同培养株（包括临床分离株）的入侵抑制作用。
儿童针对这些抗原的抗体以年龄依赖性方式增加。对不同抗原的反应广度与从成人中纯化的IgG的体外寄生虫生长抑制活性密切相关。抗体反应广度的最强预测因素是年龄和传播强度，并且对Rh2、Rh4和Rh5的反应性组合与入侵抑制密切相关。
生长抑制活性与针对裂殖子抗原的抗体反应广度显著相关，这为多组分血液期疫苗的前景提供了支持。
