Avtanski Dimiter, Chen Karin, Poretsky Leonid
Gerald J. Friedman Diabetes Institute at Lenox Hill Hospital, Northwell Health, New York, NY, USA.
Data Brief. 2019 Jun 8;25:104112. doi: 10.1016/j.dib.2019.104112. eCollection 2019 Aug.
Resistin is an adipokine produced in white adipose tissue that is thought to modulate insulin sensitivity in peripheral tissues (such as liver, skeletal muscle or adipose tissue). Human and murine resistin molecules share only about 60% sequence homology. [1] Contrary to humans, in which resistin is secreted mostly by macrophages, Park and Ahima 2013 resistin in rodents is produced primarily by the mature adipocytes of the white adipose tissue. Although resistin can bind to toll-like receptor 4 (TLF4) activating proinflammatory responses in human and rodents, [3], [4], [5], [6], [7], [8] the inflammatory actions of resistin in human monocytes were found to be mediated by resistin binding to adenylyl cyclase-associated protein 1 (CAP1). [9] In this study, we aimed to investigate the effects of resistin on the expression of various genes related to insulin resistance in mouse liver cells. Using BNL CL.2 cells, we investigated the effect of resistin in untransfected or CAP1 siRNA-transfected cells on the expression of 84 key genes involved in insulin resistance.
抵抗素是一种在白色脂肪组织中产生的脂肪因子，被认为可调节外周组织（如肝脏、骨骼肌或脂肪组织）中的胰岛素敏感性。人和小鼠的抵抗素分子序列同源性仅约为60%。[1]与人类不同，人类的抵抗素主要由巨噬细胞分泌，而2013年帕克和阿希马发现，啮齿动物中的抵抗素主要由白色脂肪组织的成熟脂肪细胞产生。尽管抵抗素可与Toll样受体4（TLR4）结合，在人和啮齿动物中激活促炎反应，[3][4][5][6][7][8]但研究发现，抵抗素在人单核细胞中的炎症作用是由抵抗素与腺苷酸环化酶相关蛋白1（CAP1）结合介导的。[9]在本研究中，我们旨在研究抵抗素对小鼠肝细胞中与胰岛素抵抗相关的各种基因表达的影响。利用BNL CL.2细胞，我们研究了抵抗素对未转染或CAP1 siRNA转染细胞中84个参与胰岛素抵抗的关键基因表达的影响。
