Salinas Z A, Babini M S, Grenat P R, Biolé F G, Martino A L, Salas N E
Ecología, Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional N° 36 - Km 601, X5804BYA Río Cuarto, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Heliyon. 2019 Jun 19;5(6):e01834. doi: 10.1016/j.heliyon.2019.e01834. eCollection 2019 Jun.
The introduction of invasive species is one of the greatest threats currently faced by natural ecosystems, causing ecological imbalances between native populations and transmission of a variety of diseases. We reported the interaction between two exotic species given by the parasitic infestation of the copepod in the early stages of the development of the American bullfrog in the central area of Argentina. In this paper we analysed the leukocyte profile of parasitized and non-parasitized tadpoles of with and their body condition (BC) as biomarkers of the health status of organisms. A total of 27 tadpoles of were analysed (12 non-parasitized and 15 parasitized). The lower BC recorded in parasitized organisms show a lower health status in these tadpoles, which could be affecting the metamorphosis and therefore impact at the population level. Leukocyte response of tadpoles to the parasitism of was found. Mature and immature lymphocyte frequencies and hematocrit were higher in parasitized compared to non-parasitized tadpoles, which is a typical response to the presence of parasites. However, eosinophils and monocytes were recorded at high frequencies in not parasitized tadpoles, which could be due to the important role played by these leucocytes in the metamorphosis of frogs. The results of this study constitute a first antecedent on leukocyte profile in aquatic stages of anurans during an ectoparasitosis and its possible implications for environmental health. The parasitism of influences the biology of the American bullfrog at both the individual and population levels. Parasitized individuals are not killed directly by the parasite, but they can create conditions for secondary infections, growth retardation, behavioral changes and, ultimately, reduce populations.
外来物种的引入是自然生态系统目前面临的最大威胁之一，会导致本地种群之间的生态失衡以及多种疾病的传播。我们报告了在阿根廷中部地区美国牛蛙发育早期，由桡足类寄生虫感染引起的两种外来物种之间的相互作用。在本文中，我们分析了感染和未感染寄生虫的牛蛙蝌蚪的白细胞谱及其身体状况（BC），将其作为生物体健康状况的生物标志物。总共分析了27只牛蛙蝌蚪（12只未感染和15只感染）。感染寄生虫的生物体中记录到的较低身体状况表明这些蝌蚪的健康状况较差，这可能会影响变态发育，进而在种群水平上产生影响。发现牛蛙蝌蚪对寄生虫感染的白细胞反应。与未感染寄生虫的蝌蚪相比，感染寄生虫的蝌蚪中成熟和未成熟淋巴细胞频率以及血细胞比容更高，这是对寄生虫存在的典型反应。然而，未感染寄生虫的蝌蚪中嗜酸性粒细胞和单核细胞的频率较高，这可能是由于这些白细胞在青蛙变态发育中发挥的重要作用。本研究结果首次揭示了无尾两栖动物水生阶段体外寄生虫感染时的白细胞谱及其对环境健康的可能影响。寄生虫感染在个体和种群水平上都会影响美国牛蛙的生物学特性。被感染的个体不会直接被寄生虫杀死，但它们会为二次感染、生长迟缓、行为改变创造条件，并最终导致种群数量减少。
