M. Liu, M. Wen, S. Shen, Prof. G. Chen, Prof. W. Zhang, Center for Soft Condensed Matter Physics, and Interdisciplinary Research & School of Physical Science, and Technology, Jiangsu Key Laboratory of Thin Films, Soochow University, Suzhou, 215006, P. R. China.
State and Local Joint Engineering Laboratory, for Novel Functional Polymeric Materials, Soochow University, Suzhou, 215123, China.
Macromol Rapid Commun. 2019 Sep;40(18):e1900215. doi: 10.1002/marc.201900215. Epub 2019 Jul 11.
Searching for vaccines (antigen and adjuvant) with easy preparation and strong T-cell response are crucial for antitumor immunity. In this work, to design lymphoseek-inspired vaccine possessing the abilities of promoting vaccine internalization and enhancing CD8 T-cell responses, a simple multicomponent strategy is successfully utilized to fabricate lymph node and dendritic cell dual-targeting glycoadjuvant@AuNPs in one pot, where three different components, catechol-containing glycopolymer, HAuCl , and amine-terminal CpG (CpG-NH ) can react in a single step to generate target adjuvant. It is found that hetero-glycoadjuvant@AuNPs could increase adjuvant internalization and enhance the activation of bone-marrow-derived dendritic cells. Critically, lymphoseek-inspired vaccine potentiates antigen-specific CD8 T-cell immune responses.
寻找具有易于制备和强 T 细胞应答的疫苗（抗原和佐剂）对于抗肿瘤免疫至关重要。在这项工作中，为了设计具有促进疫苗内化和增强 CD8 T 细胞应答能力的淋巴靶向疫苗，成功地利用了一种简单的多组分策略，一锅法制备了淋巴结和树突状细胞双重靶向糖基佐剂@AuNPs，其中三种不同的成分，含儿茶酚的糖聚合物、HAuCl 和胺端 CpG（CpG-NH ）可以在一步中反应生成靶向佐剂。研究发现，异源糖基佐剂@AuNPs 可以增加佐剂的内化并增强骨髓来源的树突状细胞的激活。至关重要的是，淋巴靶向疫苗增强了抗原特异性 CD8 T 细胞免疫应答。
