Department of Clinical Chemistry, University of Helsinki , Helsinki , Finland.
Institute for Molecular Medicine Finland (FIMM), University of Helsinki , Helsinki , Finland.
Scand J Clin Lab Invest. 2019 Oct;79(6):424-430. doi: 10.1080/00365513.2019.1639214. Epub 2019 Jul 11.
The beta subunit of human chorionic gonadotropin (hCGβ) is encoded by six genes () classified as type I and type II. mRNA is produced in large amounts by trophoblastic tissues and in small amounts by several cancerous tissues including prostate cancer and by a few benign tissues, including the prostate. Quantitative reverse-transcription polymerase chain reaction (RT-qPCR) was used to study the expression levels of all mRNAs together (total mRNA) and the two types of mRNA separately in non-cancerous ( = 74) and cancerous prostatic tissue obtained by radical prostatectomy ( = 193). RNA was isolated from formalin-fixed paraffin-embedded (FFPE) samples and mRNA levels of were correlated with disease-specific survival. Total mRNA concentrations were significantly lower ( < .0001) in cancerous than non-cancerous prostatic tissue. Separate analysis of type I and type II mRNA showed that both type I ( < .0001) and type II mRNA ( = .007) are lower in cancerous tissue than in non-cancerous tissue. Low type II mRNA level in cancerous tissue was associated with shorter cancer-specific survival ( = .001) of prostate cancer patients treated by radical prostatectomy.
人绒毛膜促性腺激素β亚基（hCGβ）由六个基因编码（），分为 I 型和 II 型。mRNA 由滋养层组织大量产生，少量由包括前列腺癌在内的几种癌组织产生，少量由包括前列腺在内的一些良性组织产生。定量逆转录聚合酶链反应（RT-qPCR）用于研究所有 mRNAs（总 mRNA）以及两种类型的 mRNA 分别在非癌性（ = 74）和通过根治性前列腺切除术获得的癌性前列腺组织（ = 193）中的表达水平。从福尔马林固定石蜡包埋（FFPE）样本中分离 RNA，并将 mRNA 水平与疾病特异性生存相关联。癌性前列腺组织中的总 mRNA 浓度明显低于非癌性前列腺组织（ < .0001）。对 I 型和 II 型 mRNA 的单独分析表明，I 型（ < .0001）和 II 型 mRNA（ = .007）在癌组织中的表达均低于非癌组织。癌组织中 II 型 mRNA 水平低与接受根治性前列腺切除术的前列腺癌患者的癌症特异性生存时间缩短相关（ = .001）。
