Rimele T J, Sturm R J, Adams L M, Henry D E, Heaslip R J, Weichman B M, Grimes D
Department of Pharmacology, Ayerst Laboratories Research, Inc., Princeton, New Jersey.
J Pharmacol Exp Ther. 1988 Apr;245(1):102-11.
Experiments were designed to study the interaction of rat peritoneal neutrophils with the vascular smooth muscle of the rat aorta. Rings of aorta, suspended in 10-ml organ chambers containing a physiologic salt solution, were precontracted with phenylephrine. Neutrophils (1 X 10(5) -4 X 10(7) cells/organ chamber) caused a cell number-dependent relaxation of the rat aorta that was augmented by superoxide dismutase (100 U/ml) or changing the oxygen content from 95 to 21%. The neutrophil-induced smooth muscle relaxation occurred in rings with and without endothelium and in rings precontracted with increasing concentrations of phenylephrine, prostaglandin F2 alpha or KCI. Catalase (1000 U/ml) and mannitol (1 X 10(-3) M) did not block the neutrophil-induced relaxation, whereas phenazine methosulfate (1 X 10(-5) M), hydroquinone (3 X 10(-5) M) and methylene blue (1 X 10(-5) M) reversed the neutrophil-induced relaxation. Pre-exposure of endothelium-rubbed rings to neutrophils (2 X 10(7) cells/organ chamber; 15 min) depressed the subsequent concentration-response curve to phenylephrine but augmented the relaxation induced by the phosphodiesterase inhibitor zaprinast (1 X 10(-5) M). The effluent from a column restraining the neutrophils induced a relaxation of endothelium-rubbed aortic rings that was prevented by methylene blue (1 X 10(-5) M). These results demonstrate that rat neutrophils release a factor that has a pharmacologic profile similar to that previously reported for the relaxing factor released from the vascular endothelium.
实验旨在研究大鼠腹膜中性粒细胞与大鼠主动脉血管平滑肌之间的相互作用。将主动脉环悬挂于含有生理盐溶液的10毫升器官浴槽中，用去氧肾上腺素使其预收缩。中性粒细胞（1×10⁵ - 4×10⁷个细胞/器官浴槽）可引起大鼠主动脉出现细胞数量依赖性舒张，超氧化物歧化酶（100 U/ml）或将氧含量从95%变为21%可增强这种舒张作用。中性粒细胞诱导的平滑肌舒张在有内皮和无内皮的主动脉环中均会发生，并且在使用浓度递增的去氧肾上腺素、前列腺素F2α或氯化钾预收缩的主动脉环中也会出现。过氧化氢酶（1000 U/ml）和甘露醇（1×10⁻³ M）不能阻断中性粒细胞诱导的舒张，而吩嗪硫酸甲酯（1×10⁻⁵ M）、对苯二酚（3×10⁻⁵ M）和亚甲蓝（1×10⁻⁵ M）可逆转中性粒细胞诱导的舒张。将去除内皮的主动脉环预先暴露于中性粒细胞（2×10⁷个细胞/器官浴槽；15分钟），会使随后对去氧肾上腺素的浓度 - 反应曲线降低，但会增强磷酸二酯酶抑制剂扎普司特（1×10⁻⁵ M）诱导的舒张。抑制中性粒细胞的柱流出液可引起去除内皮的主动脉环舒张，而亚甲蓝（1×10⁻⁵ M）可阻止这种舒张。这些结果表明，大鼠中性粒细胞释放一种因子，其药理学特性与先前报道的血管内皮释放的舒张因子相似。
