瘦素及其受体在绵羊卵巢中的免疫定位及瘦素对卵泡发育和卵母细胞成熟的体外影响。
Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.
机构信息
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil.
Laboratory of Animal Reproduction, Federal Rural University of Pernambuco, Recife, PE, Brazil.
出版信息
Mol Cell Endocrinol. 2019 Sep 15;495:110506. doi: 10.1016/j.mce.2019.110506. Epub 2019 Jul 8.
The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium.
本研究的目的是研究瘦素及其受体（LEPR）蛋白在绵羊卵巢中的免疫表达，并评估瘦素对体外培养的次级卵泡发育的影响。收集卵巢并进行免疫组织化学分析。另外收集一对卵巢，分离并培养次级卵泡，在 α-MEM 中培养 18 天，或在 10 或 25ng/ml 瘦素中培养。与所有处理相比，25ng/ml 瘦素组的腔形成和完全生长卵母细胞率更高。与 α-MEM 相比，10 或 25ng/ml 瘦素组的 GSH 水平和线粒体活性更高。25ng/ml 瘦素的 MII 比例高于 α-MEM。总之，瘦素及其受体在绵羊卵巢中表达，与对照培养基相比，25ng/ml 瘦素通过改善卵泡发育、GSH 水平和线粒体活性，提高了绵羊卵母细胞的体外成熟率。
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